Zhou Jiang, Yuan Gu
Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Chemistry. 2007;13(17):5018-23. doi: 10.1002/chem.200601605.
Electrospray ionization mass spectrometry (ESI-MS) was utilized to investigate the binding affinity and stoichiometry of small molecules with human telomeric G-quadruplex DNA. The binding-affinity order obtained for the (AGGGTT)(4) quadruplex was: Tel01>ImImImbetaDp>>PyPyPygammaImImImbetaDp. The specific binding of Tel01 and PyPyPygammaImImImbetaDp in one system consisting of human telomeric G-quadruplex and duplex DNA was observed directly for the first time. This revealed that PyPyPygammaImImImbetaDp has a binding specificity for the duplex DNA, whereas Tel01 selectively recognizes the G-quadruplex DNA. Moreover, both ESI-MS and circular dichroism (CD) spectra indicated that Tel01 favored the formation and stabilization of the antiparallel G-quadruplex, and a structural transition of the (AGGGTT)(4) sequence from a coexistence of parallel and antiparallel G-quadruplexes to a parallel G-quadruplex induced by annealing.
采用电喷雾电离质谱（ESI-MS）研究小分子与人端粒G-四链体DNA的结合亲和力和化学计量关系。对于（AGGGTT）4四链体，获得的结合亲和力顺序为：Tel01>ImImImβDp>>PyPyPyγImImImβDp。首次直接观察到在由人端粒G-四链体和双链DNA组成的一个体系中Tel01和PyPyPyγImImImβDp的特异性结合。这表明PyPyPyγImImImβDp对双链DNA具有结合特异性，而Tel01选择性识别G-四链体DNA。此外，ESI-MS和圆二色性（CD）光谱均表明Tel01有利于反平行G-四链体的形成和稳定，并且退火诱导（AGGGTT）4序列从平行和反平行G-四链体共存向平行G-四链体发生结构转变。
Zotero 插件