Contreras H R, Paredes V, Urquieta B, Del Valle L, Bustos-Obregón E
Program of Physiology and Biophysics, Institute of Biomedical Sciences. Faculty of Medicine, University of Chile, P.O.Box 70005, Postal Code 6530499, Santiago, Chile.
Biocell. 2006 Dec;30(3):423-9.
Parathion is an organophosphorate pesticide amply used in agriculture. Many alterations induced by organophosphorate pesticides have been described, such as: cytogenetic alterations in germinal cells, oligozoospermia and teratozoospermia in the mouse. The effect of Parathion, both pure (PP) and commercial (PC), on mouse interstitial cell testosterone production was evaluated in vivo and in vitro. Male mice were intraperitoneally injected with a single dose of 1/3 LD50 of Parathion, both PP and PC. The animals were sacrificed at 1, 8 and 40 days post injection to evaluate the impact of disrupting testosterone production on spermatogonia, spermatocytes and elongated spermatids. The plasma testosterone was assayed by standard radioimmunoanalysis. The same method was used to assay testosterone in the culture medium of interstitial cells obtained from the control and Parathion treated animals at the same time intervals. Sperm count, sperm teratozoospermia and tubular blockage were analyzed for an appraisal of spermatogenesis. Increase in the teratozoospermia and tubular blockage was detected in the PP and PC group at 8 and 40 days post injection. Plasma testosterone levels drop significantly at 8 days and recovered slowly at 40 days only in PP animals as detected in vivo, implying interference of testicular steroidogenesis due to the toxicant. Recuperation of normality occurs at long time intervals. In conclusion, Parathion disturbs the synthesis of testosterone in mice affecting qualitatively the spermatogenesis
对硫磷是一种在农业中大量使用的有机磷酸酯类杀虫剂。已经描述了许多由有机磷酸酯类杀虫剂引起的改变，例如：生殖细胞中的细胞遗传学改变、小鼠的少精子症和畸形精子症。评估了纯对硫磷（PP）和市售对硫磷（PC）对小鼠间质细胞睾酮生成的体内和体外影响。雄性小鼠腹腔注射单剂量为1/3 LD50的对硫磷，包括PP和PC。在注射后1、8和40天处死动物，以评估睾酮生成受干扰对精原细胞、精母细胞和延长型精子细胞的影响。通过标准放射免疫分析测定血浆睾酮。以相同方法在相同时间间隔测定从对照动物和经对硫磷处理的动物获得的间质细胞培养基中的睾酮。分析精子计数、精子畸形精子症和生精小管阻塞情况以评估精子发生。在注射后8天和40天，PP组和PC组均检测到畸形精子症增加和生精小管阻塞。在体内检测到，仅PP组动物的血浆睾酮水平在8天时显著下降，在40天时缓慢恢复，这意味着该毒物干扰了睾丸类固醇生成。长时间后恢复正常。总之，对硫磷干扰小鼠睾酮的合成，对精子发生产生定性影响。
