Rathi Ravinder, Kadian S K, Khurana Bharat, Grover Y P, Gulati B R
Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar 125 004, India.
Indian J Exp Biol. 2007 Feb;45(2):212-6.
With a view to use mice as an experimental model for studying immune response to bovine rotavirus (BRV), the kinetics of humoral and cellular immune responses to BRV in mice were evaluated by immunizing through intraperitoneal and oral route with UK strain of BRV. Following immunization with BRV, anti-rotavirus antibodies was developed in mice. The mean log antibody titres as measured by ELISA in mice immunized by intraperitoneal route were significantly higher than those immunized by oral route. Significant cellular immune response was observed in BRV-immunized mice on stimulation with BRV antigen, as measured by lymphocyte proliferation assay. The thymidine uptake by splenic and mesenteric lymph-node cells of intraperitoneally immunized mice on stimulation with BRV was 21328 +/- 1225 and 739 +/- 55 CPM, respectively. The splenic cells showed significantly higher stimulation (stimulation index 12.98) as compared to those of mesenteric cells (stimulation index 1.57). Foot pad inoculation test showed maximum virus-specific delayed type hypersensitivity reaction at 24 hr post-challenge following primary immunization and at 18 hr post-challenge following secondary immunization. The results indicate that BRV immunization by intraperitoneal route generates more efficient immune response in mice than by oral route and this route may be used for immune response studies involving BRV infection.
为了将小鼠用作研究对牛轮状病毒（BRV）免疫反应的实验模型，通过用BRV英国毒株经腹腔和口服途径免疫，评估了小鼠对BRV的体液免疫和细胞免疫反应的动力学。用BRV免疫后，小鼠体内产生了抗轮状病毒抗体。通过ELISA测量，经腹腔途径免疫的小鼠的平均对数抗体滴度显著高于经口服途径免疫的小鼠。在用BRV抗原刺激时，在经BRV免疫的小鼠中观察到显著的细胞免疫反应，通过淋巴细胞增殖试验进行测量。经腹腔免疫的小鼠的脾细胞和肠系膜淋巴结细胞在用BRV刺激时的胸苷摄取量分别为21328±1225和739±55 CPM。与肠系膜细胞相比，脾细胞显示出显著更高的刺激（刺激指数12.98）（刺激指数1.57）。足垫接种试验显示，初次免疫后攻击后24小时和二次免疫后攻击后18小时出现最大的病毒特异性迟发型超敏反应。结果表明，与口服途径相比，经腹腔途径用BRV免疫在小鼠中产生更有效的免疫反应，并且该途径可用于涉及BRV感染的免疫反应研究。
