刚地弓形虫：用编码抗原MIC2、M2AP、AMA1和BAG1的基因进行DNA疫苗接种及其免疫原性潜力评估。
Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential.
作者信息
Dautu George, Munyaka Biscah, Carmen Gabriella, Zhang Guohong, Omata Yoshitaka, Xuenan Xuan, Igarashi Makoto
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
出版信息
Exp Parasitol. 2007 Jul;116(3):273-82. doi: 10.1016/j.exppara.2007.01.017. Epub 2007 Feb 1.
A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-gamma synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes.
此前已有报道称，微线体蛋白的抗原区域组合可预防慢性弓形虫病。在本研究中，我们评估了用编码刚地弓形虫AMA1、MIC2、M2AP和BAG1蛋白序列的质粒DNA皮内免疫BALB/c和C57BL/6小鼠所诱导的免疫反应。用AMA1基因免疫的小鼠产生了高水平的血清IgG2a和c抗体以及与干扰素-γ合成相关的细胞免疫反应，提示为调节性Th1型反应。与MIC2、M2AP和BAG1基因相比，用AMA1基因免疫可对弓形虫病急性期产生部分但显著的保护作用。因此，与MIC2、M2AP和BAG1基因相比，AMA1基因似乎能产生强烈的特异性免疫反应，并对弓形虫病提供有效的保护。
Zotero 插件