大小各异病毒中微小RNA的表达与功能

Expression and function of microRNAs in viruses great and small.

作者信息

Sullivan C S, Grundhoff A, Tevethia S, Treisman R, Pipas J M, Ganem D

机构信息

Howard Hughes Medical Institute, Department of Microbiology, University of California, San Francisco, California 94143, USA.

出版信息

Cold Spring Harb Symp Quant Biol. 2006;71:351-6. doi: 10.1101/sqb.2006.71.065.

DOI:10.1101/sqb.2006.71.065

PMID:17381316

Abstract

Since they employ host gene expression machinery to execute their genetic programs, it is no surprise that DNA viruses also encode miRNAs. The small size of viral genomes, and the high degree of understanding of the functions of their gene products, make them particularly favorable systems for the examination of miRNA biogenesis and function. Here we review our computational and array-based approaches for viral miRNA discovery, and we discuss the structure and function of miRNAs identified by these approaches in polyomaviruses and herpesviruses.

摘要

由于DNA病毒利用宿主基因表达机制来执行其遗传程序，因此DNA病毒也编码微小RNA（miRNA）也就不足为奇了。病毒基因组的小尺寸以及对其基因产物功能的高度了解，使其成为研究miRNA生物发生和功能的特别有利的系统。在这里，我们回顾了我们用于发现病毒miRNA的计算和基于阵列的方法，并讨论了通过这些方法在多瘤病毒和疱疹病毒中鉴定出的miRNA的结构和功能。