Li Yun, Liu Xiaochun, Zhang Yong, Zhu Pei, Lin Haoran
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Gen Comp Endocrinol. 2007 May 15;152(1):111-22. doi: 10.1016/j.ygcen.2007.01.033. Epub 2007 Feb 9.
The cDNAs encoding two distinct growth hormone receptors (GHRs) were firstly cloned and sequenced from the liver of orange-spotted grouper (Epinephelus coioides). The cDNA of grouper GHR1 consisted of 2673-bp and encoded 658 amino acids, while the cDNA of grouper GHR2 consisted of 2989-bp and encoded 577 amino acids. The two cDNAs shared 78.6% identity in nucleotide sequence and 37.8% identity in deduced amino acid sequence. Northern blot analysis demonstrated a single GHR1 transcript of approximately 4.3 kb in liver and a single GHR2 transcript of approximately 3.9 kb in the liver and muscle. In the Real-time PCR assay, grouper GHR1 and GHR2 were expressed in all tissues tested. The expression of GHR2 was significantly higher than that of GHR1 in telencephalon, cerebellum, pituitary, heart and white muscle, whereas the expression of GHR1 was significantly higher in liver. These results indicated that there were two types of GHRs existing in orange-spotted grouper, and they had different structural features and tissues expression patterns. However, the functional differences between GHR1 and GHR2 in orange-spotted grouper remained further research.
首次从点带石斑鱼（Epinephelus coioides）肝脏中克隆并测序了编码两种不同生长激素受体（GHRs）的cDNA。石斑鱼GHR1的cDNA由2673个碱基对组成，编码658个氨基酸，而石斑鱼GHR2的cDNA由2989个碱基对组成，编码577个氨基酸。这两个cDNA在核苷酸序列上具有78.6%的同一性，在推导的氨基酸序列上具有37.8%的同一性。Northern印迹分析表明，肝脏中存在一条约4.3 kb的单一GHR1转录本，肝脏和肌肉中存在一条约3.9 kb的单一GHR2转录本。在实时PCR分析中，石斑鱼GHR1和GHR2在所有测试组织中均有表达。在端脑、小脑、垂体、心脏和白肌中，GHR2的表达显著高于GHR1，而在肝脏中，GHR1的表达显著更高。这些结果表明，点带石斑鱼中存在两种类型的GHRs，它们具有不同的结构特征和组织表达模式。然而，石斑鱼中GHR1和GHR2之间的功能差异仍有待进一步研究。
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