Kalinina Ia M
Tsitol Genet. 2006 Sep-Oct;40(5):21-7.
Using confocal microscopy the organization of tubulin cytoskeleton including endoplasmic and cortical microtubules (CMTs) has been studied in epidermal and cortical cells of the different growth zones of main root of Brassica rapa L. 6-days-old seedlings in control conditions and under clinorotation. It was shown that changes in CMTs orientation occured only in the distal elongation zone (DEZ). In the control, CMT arrays oriented transversely to the root long axis. Under clinorotation appearance of the shorter randomly organized CMTs was observed. Simultaneously, a significant decrease in the cell length in the central elongation zone (CEZ) under clinorotation was detected. It is suggested that the decline of anisotropic growth typical for CEZ cells is connected with CMTs disorientation under clinorotation.
利用共聚焦显微镜,对芜菁主根不同生长区的表皮细胞和皮层细胞中微管蛋白细胞骨架的组织情况进行了研究,该骨架包括内质微管和皮层微管(CMT)。研究对象为在对照条件下和回转处理下的6日龄幼苗。结果表明,CMT方向的变化仅发生在远端伸长区(DEZ)。在对照条件下,CMT阵列垂直于根的长轴排列。在回转处理下,观察到较短的随机排列的CMT出现。同时,检测到回转处理下中央伸长区(CEZ)细胞长度显著缩短。有人认为,CEZ细胞典型的各向异性生长的下降与回转处理下CMT的方向紊乱有关。