两相体系中有机过氧化物的化学发光检测

Chemiluminescence detection of organic peroxides in a two-phase system.

作者信息

Baj Stefan, Krawczyk Tomasz

机构信息

Department of Chemical Organic Technology and Petrochemistry, The Silesian University of Technology, ul. Krzywoustego 4, 44-100 Gliwice, Poland.

出版信息

Anal Chim Acta. 2007 Feb 28;585(1):147-53. doi: 10.1016/j.aca.2006.12.002. Epub 2006 Dec 9.

DOI:10.1016/j.aca.2006.12.002

PMID:17386659

Abstract

Potential application of chemiluminescence (CL) reaction of luminol for detection of organic peroxides by HPLC post-column reaction using an immiscible apolar eluent (hexane) with aqueous solution of luminol has been proposed. The positive influence of the addition of an anionic surface-active agent--sodium dodecyl sulphate (SDS) to the luminol solution on the CL intensity has been observed. The sensitivity of the method is greater for peroxyacid and hydroperoxide tested, and lesser for diacyl peroxide as compared to system with polar eluent miscible with solution of luminol. Our interpretation of observed results based on the extraction efficiency, CL kinetics and microemulsion formation has been suggested.

摘要

有人提出了利用鲁米诺的化学发光（CL）反应，通过高效液相色谱（HPLC）柱后反应，使用与鲁米诺水溶液不混溶的非极性洗脱剂（己烷）来检测有机过氧化物。已观察到向鲁米诺溶液中添加阴离子表面活性剂——十二烷基硫酸钠（SDS）对CL强度有积极影响。与使用与鲁米诺溶液混溶的极性洗脱剂的体系相比，该方法对所测试的过氧酸和氢过氧化物的灵敏度更高，而对二酰基过氧化物的灵敏度较低。我们基于萃取效率、CL动力学和微乳液形成对观察结果进行了解释。