Anti-hypercalcemic effect of orally administered recombinant Saccharomyces cerevisiae expressing salmon calcitonin on hypercalcemic rats.
作者信息
Sun Pingnan, Zhang Xuecheng, Zang Xiaonan, Zhou Xiaoling, Chen Yunsong, Arunakumara K K I U, Liang Bin
机构信息
College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
出版信息
Biotechnol Lett. 2007 Jul;29(7):1013-8. doi: 10.1007/s10529-007-9352-3. Epub 2007 Mar 27.
PMID:17387434
Abstract
Oral delivery of salmon calcitonin (sCT) to rats via a recombinant Saccharomyces cerevisiae was assessed. A synthetic sCT gene was cloned and expressed in S. cerevisiae yAGA2-sCT. Recombinant salmon calcitonin (rsCT) expression was detected by flow cytometry. The resorption activity of osteoclasts was inhibited by 3 x 10(-6 )M rsCT. Oral administration of 5 g lyophilized yAGA2-sCT/kg to hypercalcemic rats decreased serum calcium from 2.8 +/- 0.02-2.7 +/- 0.02 mM.
摘要
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