Selvarajan Sathiyamoorthy, Wong Ka Yan, Khoo Kei Siong, Bay Boon Huat, Tan Puay Hoon
Department of Pathology, Singapore General Hospital.
Pathology. 2006 Dec;38(6):528-33. doi: 10.1080/00313020601024060.
We aimed to investigate the immunohistochemical expression of c-erbB-2 in invasive breast carcinoma in Asian women and its correlations with clinicopathological parameters and nuclear morphometry. Patients were followed up for disease relapse and overall survival, and the data were reviewed in conjunction with c-erbB-2 over-expression.
Paraffin sections from 321 invasive breast cancers were immunohistochemically stained with anti-human c-erbB-2 antibody using the streptavidin-biotin technique.
c-erbB-2 was over-expressed in 110 (34.3%) cases, with an inverse correlation with oestrogen receptor (ER) and progesterone receptor (PR) status (p=0.0001) and a positive correlation with histological grade (p=0.017). Nuclear morphometry in 96 cases revealed rounder nuclei in c-erbB-2 negative tumours (p=0.0322) when compared with c-erbB-2 positive tumours. Among c-erbB-2 positive cases, malignant cells of histological grade 3 tumours revealed larger nuclear area and perimeter than grade 1 and 2 cases (p=0.0095, p=0.03, respectively) while increasing tumour size correlated with greater nuclear perimeter (p=0.046). c-erbB-2 positivity was significantly associated with poor survival when all patients were included in the analysis (p=0.0166) and for subsets of node positive, histological grade 1 and 2, and ER positive tumours, and in women aged over 50 years (p=0.0047, p=0.0367, p=0.0092, p=0.0096, respectively).
c-erbB-2 was independently prognostic when histological grade, nodal and ER status were considered. Our results show that c-erbB-2 over-expression correlates with poor histological grade and negative ER/PR status, and predicts poor overall survival in Asian women with breast cancer.
我们旨在研究亚洲女性浸润性乳腺癌中c-erbB-2的免疫组化表达及其与临床病理参数和核形态计量学的相关性。对患者进行疾病复发和总生存期的随访，并结合c-erbB-2过表达对数据进行回顾分析。
采用链霉亲和素-生物素技术，用抗人c-erbB-2抗体对321例浸润性乳腺癌的石蜡切片进行免疫组化染色。
110例（34.3%）病例中c-erbB-2过表达，与雌激素受体（ER）和孕激素受体（PR）状态呈负相关（p=0.0001），与组织学分级呈正相关（p=0.017）。96例病例的核形态计量学显示，与c-erbB-2阳性肿瘤相比，c-erbB-2阴性肿瘤的细胞核更圆（p=0.0322）。在c-erbB-2阳性病例中，3级组织学肿瘤的恶性细胞显示出比1级和2级病例更大的核面积和周长（分别为p=0.0095，p=0.03），而肿瘤大小增加与更大的核周长相关（p=0.046）。当所有患者纳入分析时，c-erbB-2阳性与不良生存显著相关（p=0.0166），在淋巴结阳性、组织学1级和2级、ER阳性肿瘤亚组以及50岁以上女性中也是如此（分别为p=0.0047，p=0.0367，p=0.0092，p=0.0096）。
当考虑组织学分级、淋巴结和ER状态时，c-erbB-2具有独立的预后意义。我们的结果表明，c-erbB-2过表达与不良组织学分级和ER/PR阴性状态相关，并预测亚洲乳腺癌女性的总生存期较差。
