序列特异性扩增多态性（SSAPs）：一种用于分析转座子插入的多位点方法。
Sequence-specific amplification polymorphisms (SSAPs): a multi-locus approach for analyzing transposon insertions.
作者信息
Syed Naeem H, Flavell Andrew J
机构信息
Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee DD2 5DA, UK.
出版信息
Nat Protoc. 2006;1(6):2746-52. doi: 10.1038/nprot.2006.407.
Multiple copies of transposable elements, inserted at random around the host genome, can be used as molecular markers. Sequence-specific amplification polymorphisms (SSAPs) amplify the region between a PCR primer site near the end of an element and an adjacent restriction site in the flanking genomic DNA. Each amplified insertion is revealed as a band on a sequencing gel or capillary electrophoresis, and a genomic DNA sample produces a characteristic fingerprint of bands. Here, we explain the SSAP marker development method, which can be performed for any species, with recommendations for experimental parameters in several plant species. It takes about 1-2 weeks to complete the whole SSAP marker procedure.
随机插入宿主基因组周围的多个转座元件拷贝可作为分子标记。序列特异性扩增多态性（SSAP）扩增元件末端附近的PCR引物位点与侧翼基因组DNA中相邻限制性位点之间的区域。每个扩增的插入片段在测序凝胶或毛细管电泳上显示为一条带，基因组DNA样本产生特征性的条带指纹图谱。在这里，我们解释了SSAP标记开发方法，该方法可用于任何物种，并给出了几种植物物种实验参数的建议。完成整个SSAP标记程序大约需要1-2周时间。
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