Ye Jian-Hui, Liang Yue-Rong, Jin Jing, Liang Hue-Ling, Du Ying-Ying, Lu Jian-Liang, Ye Qian, Lin Chen
Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, People's Republic of China.
J Agric Food Chem. 2007 May 2;55(9):3498-502. doi: 10.1021/jf070046c. Epub 2007 Apr 4.
The caffeine level of instant tea extracted from decaffeinated leaf tea with 4.0 mg g-1 caffeine is commonly above 10.0 mg g-1, the maximum limit of caffeine for decaffeinated instant tea. Further removal of caffeine by active carbon (AC) from the green tea extract was investigated. It showed that the removal of caffeine from the tea extract solutions depended on the treatment time and tea extract concentration while the ethanol concentration and pH had little effect on the removal of caffeine. According to the removal of caffeine and the ratio of total catechins to caffeine in the tested samples, the optimum decaffeination conditions were determined to be as follows: tea extract concentration 15-30 g L-1 for common tea extract but higher for partially decaffeinated tea leaf extract; ratio of tea solution to AC, 100 mL:4 g; treatment time, 4 h; and natural tea extract pH. Instant tea powder extracted from partially decaffeinated leaf tea with a caffeine level of 4.03 mg g-1 and further decaffeinated by AC had a caffeine level of 7.81 mg g-1, which was 31% lower than that without AC treatment.
从含4.0毫克/克咖啡因的脱咖啡因叶茶中提取的速溶茶的咖啡因含量通常高于10.0毫克/克,这是脱咖啡因速溶茶的咖啡因最大限量。研究了用活性炭(AC)进一步去除绿茶提取物中咖啡因的方法。结果表明,从茶提取物溶液中去除咖啡因取决于处理时间和茶提取物浓度,而乙醇浓度和pH值对咖啡因的去除影响很小。根据测试样品中咖啡因的去除情况以及总儿茶素与咖啡因的比例,确定最佳脱咖啡因条件如下:普通茶提取物的茶提取物浓度为15 - 30克/升,部分脱咖啡因叶茶提取物的浓度更高;茶溶液与AC的比例为100毫升:4克;处理时间为4小时;以及天然茶提取物的pH值。从含4.03毫克/克咖啡因的部分脱咖啡因叶茶中提取并经AC进一步脱咖啡因的速溶茶粉,其咖啡因含量为7.81毫克/克,比未经AC处理的速溶茶粉低31%。