肌红蛋白与聚苯乙烯磺酸盐在热解石墨上逐层组装过程中血红素释放的证据。

Evidence for heme release in layer-by-layer assemblies of myoglobin and polystyrenesulfonate on pyrolitic graphite.

作者信息

de Groot Matheus T, Merkx Maarten, Koper Marc T M

机构信息

Laboratory of Inorganic Chemistry and Catalysis, Schuit Institute of Catalysis, 5600 MB Eindhoven, The Netherlands.

出版信息

J Biol Inorg Chem. 2007 Aug;12(6):761-6. doi: 10.1007/s00775-007-0228-8. Epub 2007 Apr 5.

DOI:10.1007/s00775-007-0228-8

PMID:17410384

Abstract

Layer-by-layer assemblies of myoglobin and polystyrenesulfonate (PSS) on pyrolitic graphite have been investigated with the goal of determining the origin of the voltammetric response of these films. From the similar midpoint potential, coverage and electron transfer behavior compared with those of adsorbed free heme, it was concluded that the observed voltammetric peak is due to heme adsorbed at the electrode surface. This suggests that the interactions between the pyrolitic graphite electrode, PSS and myoglobin can result in heme release from the protein followed by heme adsorption on the electrode.

摘要

为了确定这些薄膜伏安响应的起源，对肌红蛋白和聚苯乙烯磺酸盐（PSS）在热解石墨上的逐层组装进行了研究。与吸附的游离血红素相比，从相似的中点电位、覆盖率和电子转移行为可以得出结论，观察到的伏安峰是由于血红素吸附在电极表面所致。这表明热解石墨电极、PSS和肌红蛋白之间的相互作用会导致血红素从蛋白质中释放出来，随后血红素吸附在电极上。