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两种细胞色素c2的一级结构测定:与功能不相关的线粒体细胞色素C高度相似。

Primary structure determination of two cytochromes c2: close similarity to functionally unrelated mitochondrial cytochrome C.

作者信息

Ambler R P, Meyer T E, Kamen M D

出版信息

Proc Natl Acad Sci U S A. 1976 Feb;73(2):472-5. doi: 10.1073/pnas.73.2.472.

Abstract

The amino-acid sequences of the cytochromes c2 from the photosynthetic non-sulfur purple bacteria Rhodomicrobium vannielii and Rhodopseudomonas viridis have been determined. Only a single residue deletion (at position 11 in horse cytochrome c) is necessary to align the sequences with those of mitochondrial cytochromes c. The overall sequence similarity between these cytochromes c2 and mitochondrial cytochromes c is closer than that between mitochondrial cytochromes c and the other cytochromes c2 of known sequence, and in the latter multiple insertions and deletions must be postulated before a match can be obtained. Nevertheless, these two cytochromes c2 show no better reactivity with the mitochondrial cytochrome c oxidase than do the less well-matched cytochromes c2. The bearing of these findings on possible evolutionary relationship between mitochondria and prokaryotes is discussed.

摘要

已测定了光合非硫紫色细菌凡氏红微菌和绿假单胞菌细胞色素c2的氨基酸序列。只需一个单残基缺失(在马细胞色素c的第11位),就能使这些序列与线粒体细胞色素c的序列对齐。这些细胞色素c2与线粒体细胞色素c之间的整体序列相似性,比线粒体细胞色素c与已知序列的其他细胞色素c2之间的相似性更高,而对于后者,在获得匹配之前必须假定有多个插入和缺失。然而,这两种细胞色素c2与线粒体细胞色素c氧化酶的反应性,并不比匹配度较低的细胞色素c2更好。讨论了这些发现对线粒体与原核生物之间可能的进化关系的影响。

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