Krylatov A V, Maslov L N, Ermakov S Yu, Barzakh E I, Lasukova O V, Crawford D, Ghadessy R, Serebrov V Yu
Institute of Cardiology, Siberian Division of Russian Academy of Medical Sciences, Tomsk.
Bull Exp Biol Med. 2006 Oct;142(4):450-3. doi: 10.1007/s10517-006-0389-4.
Intravenous injection of cannabinoids dissolved in cremophore EL:ethanol:NaCl mixture and water-soluble emulsion of the same cannabinoids caused identical negative chronotropic effects in chloralose-narcotized rats. Selective CB1 and CB2 receptor antagonist HU-210 also induced a negative chronotropic effect in rats, while pre-injection of CB1 receptor antagonist SR 141716A completely abolished this effect of HU-210. Selective CB2 receptor antagonist SR 144528 had no effect on HU-210-induced bradycardia. Preinjection of ganglioblocker hexamethonium also did not abolish the negative chronotropic effect of HU-210 and ACPA. Perfusion of isolated rat heart with Krebs-Henseleit solution containing HU-210 in a final concentration of 100 nM reduced heart rate. It was shown that the negative chronotropic effect of cannabinoids is mediated through activation of cardiac CB1 receptors.
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