长期乙醇摄入与偏爱酒精大鼠脑区GABAA受体亚基mRNA表达
Lifelong ethanol consumption and brain regional GABAA receptor subunit mRNA expression in alcohol-preferring rats.
作者信息
Sarviharju Maija, Hyytiä Petri, Hervonen Antti, Jaatinen Pia, Kiianmaa Kalervo, Korpi Esa R
机构信息
Department of Mental Health and Alcohol Research, National Public Health Institute, Helsinki, Finland.
出版信息
Alcohol. 2006 Nov;40(3):159-66. doi: 10.1016/j.alcohol.2006.11.003. Epub 2007 Mar 7.
Brain regional gamma-aminobutyric acid type A (GABAA) receptor subunit mRNA expression was studied in ethanol-preferring AA (Alko, Alcohol) rats after moderate ethanol drinking for up to 2 years of age. In situ hybridization with oligonucleotide probes specific for 13 different subunits was used with coronal cryostat sections of the brains. Selective alterations were observed by ethanol exposure and/or aging in signals for several subunits. Most interestingly, the putative highly ethanol-sensitive alpha4 and beta3 subunit mRNAs were significantly decreased in several brain regions. The age-related alterations in alpha4 subunit expression were parallel to those caused by lifelong ethanol drinking, whereas aging had no significant effect on beta3 subunit expression. The results suggest that prolonged ethanol consumption leading to blood concentrations of about 10 mM may downregulate the mRNA expression of selected GABAA receptor subunits and that aging might have partly similar effects.
在长达2岁的适度饮酒后，对嗜酒的AA（阿尔科，酒精）大鼠的脑区γ-氨基丁酸A型（GABAA）受体亚基mRNA表达进行了研究。使用针对13种不同亚基的寡核苷酸探针与脑的冠状低温恒温器切片进行原位杂交。通过乙醇暴露和/或衰老观察到几个亚基信号的选择性改变。最有趣的是，假定的高度乙醇敏感的α4和β3亚基mRNA在几个脑区显著降低。α4亚基表达的年龄相关变化与终身饮酒引起的变化平行，而衰老对β3亚基表达没有显著影响。结果表明，长期饮酒导致血液浓度约为10 mM可能会下调所选GABAA受体亚基的mRNA表达，并且衰老可能具有部分相似的作用。
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