Frucht D M, Lamperth L, Vicenzi E, Belcher J H, Martin M A
Laboratory of Molecular Microbiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
AIDS Res Hum Retroviruses. 1991 Sep;7(9):729-33. doi: 10.1089/aid.1991.7.729.
Previously described FVB/N mice harboring a human immunodeficiency virus (HIV) long terminal repeat (LTR)/chloramphenicol acetyl transferase (CAT) transgene were treated with varying amounts of 254 nm UV-C radiation or 312 nm UV-B radiation. At optimal exposure periods, a 20-fold increase in HIV-LTR-directed expression was observed in ear specimens collected 24 h following UV-C exposure; a fourfold increase in expression was induced by UV-B exposure. Investigation of the kinetics of UV-C induction in vivo revealed that LTR-directed gene expression began to increase 2 hours after exposure and reached a maximum on Day 3 following exposure (greater than 30-fold induction). In experiments examining the kinetics of UV-B activation, the maximum level of CAT activity in the ears of irradiated transgenic animals was fivefold above levels in unirradiated transgenic controls (Day 5). Furthermore, CAT activity was not induced in fur-bearing skin following UV exposure; however, a fourfold increase in HIV-LTR-directed expression could be elicited when hair was removed by shaving prior to UV-B treatment.
先前描述的携带人类免疫缺陷病毒（HIV）长末端重复序列（LTR）/氯霉素乙酰转移酶（CAT）转基因的FVB/N小鼠，接受了不同剂量的254纳米UV-C辐射或312纳米UV-B辐射。在最佳暴露时间段，UV-C照射后24小时采集的耳部标本中，观察到HIV-LTR指导的表达增加了20倍；UV-B照射诱导表达增加了4倍。对体内UV-C诱导动力学的研究表明，LTR指导的基因表达在照射后2小时开始增加，并在照射后第3天达到最大值（诱导超过30倍）。在检查UV-B激活动力学的实验中，照射转基因动物耳部的CAT活性最高水平比未照射的转基因对照高5倍（第5天）。此外，UV照射后有毛皮肤中未诱导出CAT活性；然而，在UV-B处理前通过剃毛去除毛发时，HIV-LTR指导的表达可增加4倍。
