关于28S rRNA的α-肌动蛋白茎环结构
On the conformation of the alpha sarcin stem-loop of 28S rRNA.
作者信息
Szewczak A A, Chan Y L, Moore P B, Wool I G
机构信息
Department of Chemistry, Yale University, New Haven, CT 06511.
出版信息
Biochimie. 1991 Jul-Aug;73(7-8):871-7. doi: 10.1016/0300-9084(91)90128-n.
A synthetic RNA that is a substrate for the cytotoxin alpha sarcin has been examined by NMR. The molecule in question includes the entire sequence of the so-called alpha sarcin loop from rat 28S rRNA (U4316-C4332), and it is cleaved at the residue that corresponds to G4325, the site of alpha sarcin cleavage in 28S rRNA. The data show that the terminal stem designed into the molecule's sequence exists, as expected, and that its loop has a definite structure, which is stable to at least 40 degrees C under ionic conditions compatible with its cleavage by alpha sarcin.
一种作为细胞毒素α-肌动蛋白底物的合成RNA已通过核磁共振（NMR）进行了研究。所讨论的分子包含大鼠28S rRNA（U4316 - C4332）中所谓的α-肌动蛋白环的完整序列，并且在对应于G4325的残基处被切割，该位点是28S rRNA中α-肌动蛋白的切割位点。数据表明，设计在分子序列中的末端茎如预期那样存在，并且其环具有确定的结构，在与α-肌动蛋白切割相容的离子条件下，该结构至少在40摄氏度时是稳定的。
Zotero 插件