Zhang Gui-xiang, Li Yu-jie, Zhang Feng, Zhao Jing-long, Li Kang-an, Hu Yun-sheng
Department of Radiology Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai 200080, China.
Zhonghua Yi Xue Za Zhi. 2007 Jan 23;87(4):228-32.
To monitor the effects of labeling C6 rat glioma cells with different concentrations of USPIO in vivo and in vitro.
C6 rat glioma cells of 1 x 10(6), 2 x 10(6) and 1 x 10(7) were labeled with 0 microg/ml, 25 microg/ml, 50 microg/ml USPIO, The signal intensity of cells were evaluated by MRI with T(1)WI, T(2)WI and GRE/30 degrees sequences in vitro. 1 x 10(6) of C6 glioma cells were labeled with 0 microg/ml, 25 microg/ml, 50 microg/ml USPIO and inoculated into the right frontal lobe of 2 rats under stereotaxis apparatus respectively (total 6 rats), Same MRI parameters were used just as above. Iron particle density and cells was measured by HE and Prussian blue stain under microscopy.
Different cell population was cultured with 0 microg/ml, 25 microg/ml, 50 microg/ml USPIO about 12 hours. The MR signal intensity of labeling cells were inversely correlated with the different concentration of USPIO groups in T(2)W and GRE/30 degrees imaging (t = 4.19, 3.38, P < 0.05) in vitro. There was an inversely correlation between the labeling cell population and the signal intensity at the same concentration of USPIO (t = 5.16, 2.35, 4.41; P < 0.05). Dyeing degree of labeling cells stained by Prussian blue gradually deepened from 25 microg/ml to 50 microg/ml by microscopy. In vivo MRI can clearly show the cells labeled with 25 microg/ml USPIO.
Iron particle density in the rat glioma cells were gradually increased with the concentration of USPIO. The MR signal intensity was inversely correlated with the cell population at the same condition. 25 microg/ml USPIO labeling rat glioma cells were enough for in vivo monitoring by MRI.
监测不同浓度超顺磁性氧化铁（USPIO）标记C6大鼠胶质瘤细胞的体内外效果。
分别用0微克/毫升、25微克/毫升、50微克/毫升USPIO标记1×10⁶、2×10⁶和1×10⁷的C6大鼠胶质瘤细胞，体外通过磁共振成像（MRI）的T1加权成像（T1WI）、T2加权成像（T2WI）和梯度回波/30°序列（GRE/30°）评估细胞的信号强度。将1×10⁶的C6胶质瘤细胞分别用0微克/毫升、25微克/毫升、50微克/毫升USPIO标记，然后在立体定位仪下分别接种到2只大鼠的右侧额叶（共6只大鼠），采用与上述相同的MRI参数。通过苏木精-伊红（HE）染色和普鲁士蓝染色在显微镜下测量铁颗粒密度和细胞数量。
不同细胞数量分别用0微克/毫升、25微克/毫升、50微克/毫升USPIO培养约12小时。体外T2WI和GRE/30°成像中，标记细胞的磁共振信号强度与不同浓度的USPIO组呈负相关（t = 4.19，3.38，P < 0.05）。在相同浓度的USPIO下，标记细胞数量与信号强度呈负相关（t = 5.16，2.35，4.41；P < 0.05）。显微镜下观察，普鲁士蓝染色的标记细胞染色程度从25微克/毫升到50微克/毫升逐渐加深。体内MRI能清晰显示用25微克/毫升USPIO标记的细胞。
大鼠胶质瘤细胞中铁颗粒密度随USPIO浓度的增加而逐渐升高。在相同条件下，磁共振信号强度与细胞数量呈负相关。25微克/毫升USPIO标记大鼠胶质瘤细胞足以用于MRI体内监测。
