Xu Ai-fang, Wang Miao-chan, Sui Dong-ming, Yuan You-hong, Chen Gang, Lou Guo-qiang
The Sixth People's Hospital of Hangzhou, Hangzhou 310014, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Mar;21(1):67-9.
To evaluate the usefulness of new microspincolumn method for the measurement of a1pha-fetoprotein variant AFP-L3 in differentiation of benign and malignant liver disease and the warming for liver cancer.
AFP-L3 was isolated by using microspincolumn coupled with lens culinaris agglutinin (LCA), AFP and AFP-L3 were determined with chemiluminescent immunoassay, the proportion of AFP-L3 levels AFP-L3(%) were calculated, and the relationship between the elevated AFP-L3(%) levels and benign and malignant liver disease was analyzed.
The levels of AFP-L3(%) in serum of patients with hepatocellular carcinoma was significantly higher than those in the patients with other liver diseases (P < 0.001). Taking AFP-L3(%) >or= 10% as the diagnostic criteria, the sensitivity for diagnosis of liver cancer was 90.9%.
Detection of AFP-L3 seemed to be of clinical value in diagnosis and differential diagnosis of hepatocellular carcinoma; it may be especially important for identifying patients with hepatocellular carcinoma whose a1pha-fetoprotein level is low.
评估新型微柱法检测甲胎蛋白变异体AFP-L3在鉴别良恶性肝病及肝癌预警方面的应用价值。
采用微柱法结合刀豆球蛋白A（LCA）分离AFP-L3，运用化学发光免疫分析法测定AFP及AFP-L3，计算AFP-L3水平占AFP的比例（AFP-L3%），分析AFP-L3（%）水平升高与良恶性肝病之间的关系。
肝细胞癌患者血清中AFP-L3（%）水平显著高于其他肝病患者（P<0.001）。以AFP-L3（%）≥10%为诊断标准，诊断肝癌的敏感度为90.9%。
检测AFP-L3对肝细胞癌的诊断及鉴别诊断具有临床价值；对甲胎蛋白水平较低的肝细胞癌患者的识别可能尤为重要。
