猪分化簇14（CD14）基因的分子克隆、定位及组织表达

Molecular cloning, mapping, and tissue expression of the porcine cluster of differentiation 14 (CD14) gene.

作者信息

Qiu Xiao-Tian, Li Yu-Hua, Li Hejun, Yu Ying, Zhang Qin

机构信息

Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, PR China.

出版信息

Biochem Genet. 2007 Jun;45(5-6):459-68. doi: 10.1007/s10528-007-9088-8. Epub 2007 Apr 18.

DOI:10.1007/s10528-007-9088-8

PMID:17440807

Abstract

CD14 plays an important role in initiating the innate response to lipopolysaccharide from Gram-negative bacteria. The gene and corresponding cDNA of porcine CD14 were sequenced and characterized. The porcine CD14 gene consists of two exons and a short intron (80 bp) located immediately after the ATG translation start codon. This structure is very similar to the CD14 gene of human, rat, mouse, rabbit, horse, and cow. The sequence of the porcine CD14 protein is 59-76% identical to that of rat, mouse, rabbit, human, horse, and cow CD14 protein. A highly conserved structure of the CD14 protein with respect to the leucine-rich repeats domain and the N-glycosylation sites was observed between species. Porcine CD14 was assigned to porcine chromosome 2q21 by a radiation hybrid panel. Using RT-PCR analysis, porcine CD14 transcripts were detected in liver, spleen, thymus, white matter, and skeletal muscle.

摘要

CD14在启动对革兰氏阴性菌脂多糖的天然免疫反应中发挥重要作用。对猪CD14的基因和相应的cDNA进行了测序和特征分析。猪CD14基因由两个外显子和一个位于ATG翻译起始密码子之后的短内含子（80bp）组成。这种结构与人、大鼠、小鼠、兔、马和牛的CD14基因非常相似。猪CD14蛋白的序列与大鼠、小鼠、兔、人、马和牛CD14蛋白的序列有59%-76%的同一性。在不同物种之间观察到CD14蛋白在富含亮氨酸重复序列结构域和N-糖基化位点方面具有高度保守的结构。通过辐射杂种板将猪CD14定位到猪的2号染色体q21区域。使用逆转录聚合酶链反应（RT-PCR）分析，在肝脏、脾脏、胸腺、白质和骨骼肌中检测到猪CD14转录本。