金属蛋白酶9是凋亡角质形成细胞中桥粒芯糖蛋白3的外在执行者。
Metalloproteinase 9 is the outer executioner of desmoglein 3 in apoptotic keratinocytes.
作者信息
Cirillo N, Femiano F, Gombos F, Lanza A
机构信息
Regional Center on Craniofacial Malformations-MRI, Department of Odontostomatology, 1st School of Medicine and Surgery, II University of Naples, Naples, Italy.
出版信息
Oral Dis. 2007 May;13(3):341-5. doi: 10.1111/j.1601-0825.2006.01287.x.
OBJECTIVE
To investigate the specific matrix metalloproteinases (MMPs) targeting desmoglein 3 (Dsg3) in apoptotic keratinocytes.
METHOD
Inhibitor studies on cultured keratinocytes and Western blot analysis.
RESULTS
Blocking of MMP-9 activity strongly reduces shedding of Dsg3 from cell surface. MMP-2 has a less relevant role in the cleavage of Dsg3 while other MMPs, such as MMP-1, -3, and -8, do not target Dsg3.
CONCLUSION
Apoptic keratinocytes impair the extracellular domain of cell surface Dsg3 by MMP-9 activity. The discovery of a specific targeting of Dsg3 could be useful to understand the pathophysiology of diseases in which Dsg3 is affected.
目的
研究凋亡角质形成细胞中靶向桥粒芯糖蛋白3（Dsg3）的特定基质金属蛋白酶（MMPs）。
方法
对培养的角质形成细胞进行抑制剂研究及蛋白质印迹分析。
结果
抑制MMP-9活性可显著减少Dsg3从细胞表面的脱落。MMP-2在Dsg3的裂解中作用较小，而其他MMPs，如MMP-1、-3和-8，并不靶向Dsg3。
结论
凋亡角质形成细胞通过MMP-9活性破坏细胞表面Dsg3的细胞外结构域。发现Dsg3的特异性靶向可能有助于理解Dsg3受影响疾病的病理生理学。
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