Chae Jong-Chan, Kim Eungbin, Bini Elisabetta, Zylstra Gerben J
Biotechnology Center for Agriculture and Environment, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901-8520, USA.
Biochem Biophys Res Commun. 2007 Jun 8;357(3):815-9. doi: 10.1016/j.bbrc.2007.04.027. Epub 2007 Apr 16.
A catechol 2,3-dioxygenase (C23O) gene was found from Sulfolobus solfataricus strain 98/2. Heterologous thermophilic C23O expressed in Escherichia coli showed the highest activity against catechol and 4-chlorocatechol, and at neutral pH. The C23O gene located with a putative multicomponent monooxygenase (MM) gene cluster that exactly matched with the homologous region of S. solfataricus strain P2. Primary sequence comparison identified an insertion sequence (IS) element inserted into a putative MM protein A N-terminal fragment gene in strain 98/2. Both ends of the transposase gene in the IS element, ISC1234, were flanked by 19 bp inverted repeat and 4 bp direct repeat sequences which are typical features of mobile elements. Our analysis and the two geographically distant origins of strains 98/2 and P2 (USA and Italy, respectively) suggest that the two strains have evolved from a common ancestor.
从嗜热栖热菌98/2菌株中发现了一种儿茶酚2,3 -双加氧酶（C23O）基因。在大肠杆菌中表达的异源嗜热C23O对儿茶酚和4 -氯儿茶酚表现出最高活性，且在中性pH条件下活性最高。C23O基因与一个假定的多组分单加氧酶（MM）基因簇位于一起，该基因簇与嗜热栖热菌P2菌株的同源区域完全匹配。一级序列比较确定在98/2菌株中一个假定的MM蛋白A N端片段基因中插入了一个插入序列（IS）元件。IS元件ISC1234中转座酶基因的两端侧翼分别为19 bp反向重复序列和4 bp直接重复序列，这是移动元件的典型特征。我们的分析以及98/2和P2菌株在地理上的两个遥远起源（分别来自美国和意大利）表明，这两个菌株是由一个共同祖先进化而来的。
