用表达棉尾兔乳头瘤病毒（CRPV）L2E7E2基因的重组卡介苗免疫的兔子可使已形成的乳头瘤消退。

Rabbits immunised with recombinant BCG expressing the cottontail rabbit papillomavirus (CRPV) L2E7E2 genes induces regression of established papillomas.

作者信息

Govan V A, Williamson A-L

机构信息

Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town, South Africa.

出版信息

Virus Res. 2007 Jul;127(1):43-8. doi: 10.1016/j.virusres.2007.03.016. Epub 2007 Apr 23.

DOI:10.1016/j.virusres.2007.03.016

PMID:17451831

Abstract

We previously demonstrated in a cottontail rabbit papillomavirus (CRPV) challenge model that recombinant Bacille Calmette-Guerin (rBCG) could potentially be used as a prophylactic vaccine vehicle to deliver papillomavirus proteins. In this study we investigated whether regression of CRPV-induced papillomas could be achieved following immunisation of out-bred New Zealand White rabbits with rBCG expressing CRPVL2, CRPVE2, CRPVE7 or CRPVL2E7E2 proteins. Rabbits immunised with rBCG/CRPVL2E7E2 had papillomas that were largely suppressed and were significantly smaller compared to the rBCG negative control group (P</=0.01). In addition, four of the six rabbits immunised with rBCG/CRPVL2E7E2 had papillomas that completely regressed 1.5 weeks post third immunisation. Rabbits immunised with rBCG/CRPVL2, rBCG/CRPVE7, or rBCG/CRPVE2 had papillomas that were significantly smaller than the negative control rabbits (P</=0.05). The findings in this study suggest that BCG could probably be used as a vaccine delivery vehicle for human papillomavirus proteins as a possible therapeutic vaccine.

摘要

我们之前在棉尾兔乳头瘤病毒（CRPV）攻击模型中证明，重组卡介苗（rBCG）有潜力用作递送乳头瘤病毒蛋白的预防性疫苗载体。在本研究中，我们调查了用表达CRPV L2、CRPV E2、CRPV E7或CRPV L2E7E2蛋白的rBCG免疫远交新西兰白兔后，是否能实现CRPV诱导的乳头瘤消退。用rBCG/CRPV L2E7E2免疫的兔子的乳头瘤大部分受到抑制，与rBCG阴性对照组相比明显更小（P≤0.01）。此外，用rBCG/CRPV L2E7E2免疫的6只兔子中有4只在第三次免疫后1.5周乳头瘤完全消退。用rBCG/CRPV L2、rBCG/CRPV E7或rBCG/CRPV E2免疫的兔子的乳头瘤明显小于阴性对照兔子（P≤0.05）。本研究结果表明，卡介苗可能用作人乳头瘤病毒蛋白的疫苗递送载体，作为一种可能的治疗性疫苗。