Jin Yide, Hernandez Ana, Fuller Laphalle, Rosen Anne, Cirocco Robert, Esquenazi Violet, Ciancio Gaetano, Burke George W, Miller Joshua
Lillian Jean Kaplan Renal Transplant Center of the Division of Transplantation of the Department of Surgery, University of Miami, Leonard M. Miller School of Medicine, Miami, Florida 33101, USA.
Hum Immunol. 2007 May;68(5):350-61. doi: 10.1016/j.humimm.2006.11.005. Epub 2006 Dec 22.
Renal transplant rejection and graft versus host reactions between HLA genetically-identical sibling (HLAgi) donor/recipient (D/R) pairs are thought to result from minor histocompatibility antigen (mHAg) disparities. We have compared two methods of measuring HLAgi D/R T lymphocyte responses to "matured" dendritic cells: 1.) a modified Cylex assay of CD4(+) ATP levels (MLDC-ATP) versus 2.) (3)H-thymidine uptake (MLDC-(3)H). The MLDC-ATP kinetics peaked at 48 hours versus the MLDC-(3)H at 7 days, and appeared more sensitive. We tested HLAgi (normal) volunteer siblings (NLs), and D/R sibling pairs before and after renal transplantation (pre-Tx and post-Tx). The overall frequencies of positive responses in the MLDC-ATP for HLAgi NLs, pre-Tx, and post-Tx D/R pairs were 63%, 50%, and 42%, respectively. The percentage with reciprocal responses was 37.5%, 20%, and 22.22%, respectively. In one set of three HLAgi (NLs) siblings (two males and one female), there was a nongender-associated differential response. There was no MLDC correlation with class I MHC-associated mHAg (SSP) incompatibility, nor could some MLDC positive reactive pairs theoretically process the necessary HLA-class I restriction molecules for presentation of known (nanomeric) mHAg peptides. Speculatively, the MLDC reflects class II MHC-restricted mHAg reactions (not yet definable), with possible effects of other polymorphic (nonhistocompatibility) immune response genes, and thereby may be a useful measurement of CD4(+) T-cell HLAgi transplantation immunity.
在人类白细胞抗原（HLA）基因相同的同胞供体/受体（D/R）对之间，肾移植排斥反应和移植物抗宿主反应被认为是由次要组织相容性抗原（mHAg）差异引起的。我们比较了两种测量HLA基因相同的D/R T淋巴细胞对“成熟”树突状细胞反应的方法：1.）一种改良的Cylex法检测CD4(+) ATP水平（MLDC - ATP），与2.）（3）H - 胸苷摄取法（MLDC - （3）H）。MLDC - ATP动力学在48小时达到峰值，而MLDC - （3）H在7天达到峰值，并且似乎更敏感。我们检测了HLA基因相同的（正常）志愿者同胞（NLs），以及肾移植前后（移植前和移植后）的D/R同胞对。HLA基因相同的NLs、移植前和移植后的D/R对在MLDC - ATP中阳性反应的总体频率分别为63%、50%和42%。相互反应的百分比分别为37.5%、20%和22.22%。在一组三个HLA基因相同的（NLs）同胞（两男一女）中，存在与性别无关的差异反应。MLDC与I类主要组织相容性复合体相关的mHAg（SSP）不相容性无相关性，从理论上讲，一些MLDC阳性反应对也无法处理已知（纳米级）mHAg肽呈递所需的I类HLA限制性分子。推测地，MLDC反映了II类主要组织相容性复合体限制的mHAg反应（尚未明确），可能受到其他多态性（非组织相容性）免疫反应基因的影响，因此可能是一种有用的测量CD4(+) T细胞HLA基因相同移植免疫的方法。
