Oku K, Tanaka A, Yamanishi H, Nishizawa Y, Matsumoto K, Shiozaki H, Mori T
Department of Pathology, Osaka University Medical School, Japan.
Anticancer Res. 1991 Jul-Aug;11(4):1591-5.
In order to investigate molecular mechanisms of the growth of human esophageal cancer in relation to growth factors, we have recently established a protein-free culture system [Ham's F-12: Eagle's minimum essential medium (1:1, v/v)] of TE-3-OS cells (a cloned cell line from human esophageal squamous cancer, TE-3). In the present study, we first examined effects of exogenous growth factors on the growth of TE-3-OS cells. The growth of TE-3-OS cells in the protein-free medium was significantly stimulated by insulin and insulin-like growth factor (IGF)-I or IGF-II, and less effectively stimulated by epidermal growth factor (EGF) or transforming growth factor (TGF)-alpha; platelet-derived growth factor, TFG-beta, acidic fibroblast growth factor (FGF) or basic FGF had no effects. TE-3-OS cells contained specific IGF-I binding sites (110,000 sites/cell), with a Kd value of 800 pM. Moreover, the growth induced by IGF-I, IGF-II or insulin was markedly and similarly (70-80%) inhibited by anti-IGF-I receptor antibody IgG. These data suggest that IGF-I, IGF-II and insulin, as well as EGF and TGF-alpha, are important mitogens for human esophageal cancer cells and that effects of IGFs and insulin are mediated predominantly via IGF-I receptors.
为了研究人类食管癌生长与生长因子相关的分子机制，我们最近建立了TE-3-OS细胞（一种源自人食管鳞状癌TE-3的克隆细胞系）的无蛋白培养系统[Ham's F-12：伊格尔最低必需培养基（1:1，v/v）]。在本研究中，我们首先检测了外源性生长因子对TE-3-OS细胞生长的影响。在无蛋白培养基中，胰岛素、胰岛素样生长因子（IGF）-I或IGF-II能显著刺激TE-3-OS细胞的生长，表皮生长因子（EGF）或转化生长因子（TGF）-α的刺激作用较弱；血小板衍生生长因子、TGF-β、酸性成纤维细胞生长因子（FGF）或碱性FGF则无作用。TE-3-OS细胞含有特异性IGF-I结合位点（110,000个位点/细胞），解离常数（Kd）值为800 pM。此外，抗IGF-I受体抗体IgG能显著且相似地（70-80%）抑制IGF-I、IGF-II或胰岛素诱导的生长。这些数据表明，IGF-I、IGF-II和胰岛素，以及EGF和TGF-α是人类食管癌细胞重要的促有丝分裂原，且IGF和胰岛素的作用主要通过IGF-I受体介导。
