Desgranges C, Larrue J, Bricaud H
C R Seances Soc Biol Fil. 1975;169(3):544-7.
Cells obtained from the media and intima of ten days rat aorta, after enzymatic dissociation, were grown in subculture for up to three months. Electron microscopic observations demonstrate that these cells maintained the morphology of smooth muscle cells at all phases of their growth in subculture and kept their ability to synthesize and secrete intracellular proteins with better enzymatic features than the cells obtained by proliferation at the periphery of an explant.
从10日龄大鼠主动脉中膜和内膜获取的细胞,经酶解后进行传代培养,培养时间长达三个月。电子显微镜观察表明,这些细胞在传代培养的各个生长阶段均保持平滑肌细胞的形态,并保持其合成和分泌细胞内蛋白质的能力,且与从外植体周边增殖获得的细胞相比,具有更好的酶学特性。