Davies Mark R, McMillan David J, Beiko Robert G, Barroso Vanessa, Geffers Robert, Sriprakash Kadaba S, Chhatwal Gursharan S
Bacterial Pathogenesis Laboratory, The Queensland Institute of Medical Research, Australia.
Clin Infect Dis. 2007 Jun 1;44(11):1442-54. doi: 10.1086/516780. Epub 2007 Apr 19.
In spite of the emerging importance of Streptococcus dysgalactiae subspecies equisimilis (human group C streptococci [GCS] and group G streptococci [GGS]) in human health, its molecular makeup remains largely undefined. Apart from sharing a phylogenetic relationship with the human pathogen group A streptococci (GAS), GCS/GGS and GAS colonize the same ecological niche and exhibit considerable overlap in their disease profiles. Such similarities imply that the virulence factors associated with diseases may also be similar.
In this study, we used a targeted microarray containing 216 GAS virulence genes to profile the virulence gene repertoires of 58 S. dysgalactiae subspecies equisimilis isolates recovered during human infections. We performed comparative analyses to investigate the relationship between GAS virulence genes in and the invasive potential of GCS/GGS.
Up to one-half of the GAS virulence genes represented in the microarray were identified in GCS/GGS. No statistical differences were observed between isolates harboring the group C versus group G carbohydrates; however, clustering algorithms revealed 2 genetically distinct clusters of S. dysgalactiae subspecies equisimilis isolates. No relationship was observed between the virulence profile of GCS/GGS and the propensity for disease or the tissue site of isolation.
This is, to our knowledge, the first comprehensive analysis of the virulence profile of S. dysgalactiae subspecies equisimilis, and it enables novel insights into the pathogen's genetic basis of disease propensity shared with GAS. Human group C and group G streptococci may not be considered to be separate species; in fact, they may constitute 2 distinct lineages. Additional incongruent relationships were observed between virulence profiles and GCS/GGS disease propensity.
尽管停乳链球菌马链球菌亚种（人类C组链球菌[GCS]和G组链球菌[GGS]）在人类健康中的重要性日益凸显，但其分子组成在很大程度上仍不明确。除了与人类病原体A组链球菌（GAS）具有系统发育关系外，GCS/GGS和GAS定殖于相同的生态位，并且在疾病谱方面表现出相当大的重叠。这些相似性表明与疾病相关的毒力因子可能也相似。
在本研究中，我们使用了一个包含216个GAS毒力基因的靶向微阵列，对在人类感染期间分离出的58株停乳链球菌马链球菌亚种菌株的毒力基因库进行分析。我们进行了比较分析，以研究GAS毒力基因与GCS/GGS侵袭潜力之间的关系。
在微阵列中所代表的GAS毒力基因中，高达一半在GCS/GGS中被鉴定出来。携带C组与G组碳水化合物的菌株之间未观察到统计学差异；然而，聚类算法揭示了停乳链球菌马链球菌亚种菌株的2个基因不同的簇。未观察到GCS/GGS的毒力谱与疾病倾向或分离组织部位之间的关系。
据我们所知，这是对停乳链球菌马链球菌亚种毒力谱的首次全面分析，它使我们能够对该病原体与GAS共有的疾病倾向的遗传基础有新的认识。人类C组和G组链球菌可能不应被视为不同的物种；事实上，它们可能构成2个不同的谱系。在毒力谱与GCS/GGS疾病倾向之间还观察到其他不一致的关系。
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