Liu Jingying, Ma Pengda, Sun Yan, Yang Meiying, Yang Liping, Li Yuxi, Wu Yanfang, Zhu Xiaojuan, Wang Xingzhi
Institute of Genetics and Cytology, Northeast Normal University, 5268 Renmin Street, Changchun 130024, People's Republic of China.
Biotechnol Appl Biochem. 2007 Nov;48(Pt 3):143-7. doi: 10.1042/BA20070004.
The aFGF (acidic fibroblast growth factor) plays an important role in morphogenesis, angiogenesis and wound healing and is therefore of potential medical interest. A DNA fragment encoding haFGF (human aFGF) has been cloned into the PVX (potato virus X)-based binary vector (pgR107) and transiently expressed in Nicotiana benthamiana (a wild Australian tobacco) by agroinfection. Approx. 1 week after agroinfection, the recombinant haFGF accumulated in the agroinfected plants reached up to 1% of the total soluble protein. haFGF was then purified on heparin-Sepharose CL-6B. The purified haFGF could stimulate the growth of NIH 3T3 cells, suggesting that the recombinant haFGF expressed via PVX viral vector in N. benthamiana was active biologically.
酸性成纤维细胞生长因子（aFGF）在形态发生、血管生成和伤口愈合中发挥着重要作用，因此具有潜在的医学研究价值。一个编码人酸性成纤维细胞生长因子（haFGF）的DNA片段已被克隆到基于马铃薯X病毒（PVX）的双元载体（pgR107）中，并通过农杆菌介导的感染在本氏烟草（一种澳大利亚野生烟草）中瞬时表达。农杆菌介导的感染大约1周后，在被农杆菌感染的植物中积累的重组haFGF达到总可溶性蛋白的1%。然后，haFGF在肝素-琼脂糖CL-6B上进行纯化。纯化后的haFGF能够刺激NIH 3T3细胞的生长，这表明通过PVX病毒载体在本氏烟草中表达的重组haFGF具有生物学活性。
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