Rakhimberdieva Marina G, Bolychevtseva Yulia V, Elanskaya Irina V, Karapetyan Navassard V
A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, 119071 Moscow, Russia.
FEBS Lett. 2007 May 29;581(13):2429-33. doi: 10.1016/j.febslet.2007.04.056. Epub 2007 Apr 30.
An inquiry into the effect of temperature on carotenoid triggered quenching of phycobilisome (PBS) fluorescence in a photosystem II-deficient mutant of Synechocystis sp. results in identification of two temperature-dependent processes: one is responsible for the quenching rate, and one determines the yield of PBS fluorescence. Non-Arrhenius behavior of the light-on quenching rate suggests that carotenoid-absorbed light triggers a process that bears a strong resemblance to soluble protein folding, showing temperature-dependent enthalpy of activated complex formation. The response of PBS fluorescence yield to hydration changing additives and to passing of the membrane lipid phase transition point indicates that the pool size of PBSs subject to quenching depends on the state of some membrane component.
对温度对集胞藻属光系统II缺陷型突变体中类胡萝卜素引发的藻胆体（PBS）荧光猝灭的影响进行的一项研究，确定了两个与温度相关的过程：一个负责猝灭速率，另一个决定PBS荧光产量。光照猝灭速率的非阿累尼乌斯行为表明，类胡萝卜素吸收的光引发了一个与可溶性蛋白质折叠极为相似的过程，该过程显示出活化络合物形成的温度依赖性焓变。PBS荧光产量对水合变化添加剂以及膜脂质相变点通过的响应表明，遭受猝灭的PBS的库大小取决于某些膜成分的状态。
