转移相关蛋白1在人和小鼠成熟睾丸中的免疫定位评估及其与精子发生的关系。

Immunolocalization assessment of metastasis-associated protein 1 in human and mouse mature testes and its association with spermatogenesis.

作者信息

Li Wei, Liu Xin-Ping, Xu Ruo-Jun, Zhang Yuan-Qiang

机构信息

Department of Histology and Embryology, The Fourth Military Medical University, Xi'an 710032, China.

出版信息

Asian J Androl. 2007 May;9(3):345-52. doi: 10.1111/j.1745-7262.2007.00245.x.

DOI:10.1111/j.1745-7262.2007.00245.x

PMID:17486275

Abstract

AIM

To investigate the stage-specific localization of metastasis-associated protein 1 (MTA1) during spermatogenesis in adult human and mouse testis.

METHODS

The immunolocalization of MTA1 was studied by immunohistochemistry and Western blot analysis. The distribution pattern of MTA1 in mouse testis was confirmed by using quantitative analysis of purified spermatogenic cells.

RESULTS

The specificity of polyclonal antibody was confirmed by Western blot analysis. MTA1 was found expressed in the nucleus of germ cells, except elongate spermatids, and in the cytoplasm of Sertoli cells; Leydig cells did not show any specific reactivity. MTA1 possessed different distribution patterns in the two species: in humans, the most intensive staining was found in the nucleus of round spermatids and of primary spermatocytes while in mice, the most intense MTA1 staining was in the nucleus of leptotene, zygotene and pachytene spermatocytes. In both species the staining exhibited a cyclic pattern.

CONCLUSION

The present communication initially provides new evidence for the potential role of MTA1 in mature testis. In addition, its distinctive expression in germ cells suggests a regulatory role of the peptide during spermatogenesis.

摘要

目的

研究转移相关蛋白1（MTA1）在成年人类和小鼠睾丸生精过程中的阶段特异性定位。

方法

采用免疫组织化学和蛋白质印迹分析研究MTA1的免疫定位。通过对纯化的生精细胞进行定量分析，证实MTA1在小鼠睾丸中的分布模式。

结果

蛋白质印迹分析证实了多克隆抗体的特异性。发现MTA1在除伸长型精子细胞外的生殖细胞核以及支持细胞的细胞质中表达；睾丸间质细胞未显示任何特异性反应。MTA1在这两个物种中具有不同的分布模式：在人类中，最强烈的染色出现在圆形精子细胞和初级精母细胞的细胞核中，而在小鼠中，MTA1染色最强烈的是细线期、偶线期和粗线期精母细胞的细胞核。在这两个物种中，染色均呈现周期性模式。