辐射损伤会暂时诱导主动脉-性腺-中肾（AGM）来源的基质细胞中APN/CD13肽酶活性增强。

Irradiation injury temporarily induces enhancement of APN/CD13 peptidase activity on aorta-gonads-mesonephros (AGM)-derived stromal cells.

作者信息

Zhu Yan, Huang Lifang, Luo Xiaohua, Sun Hanying, Ran Dan, Zhang Kejie, Zheng Miao, Zhou Kun, Liu Wenli

机构信息

Department of Hematology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, China.

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2007 Apr;27(2):145-8. doi: 10.1007/s11596-007-0209-8.

DOI:10.1007/s11596-007-0209-8

PMID:17497281

Abstract

This study was designed to investigate the expression of aminopeptidase N (APN)/CD13 on intraembryonic AGM stromal cells, and the change of its enzymatic activity after irradiation injury. The expression of APN/CD13 on AGM stromal cells was assayed by RT-PCR and immunohistochemistry. After the stromal cells in AGM region were irradiated with 8.0 Gy of (60)Co gamma-rays, APN/CD13 enzymatic activity was measured by spectrophotometer at different time points. The result showed that AGM stromal cells strongly expressed APN/CD13. The enzymatic activity of APN/CD13 decreased temporarily after irradiation injury, then increased to higher level 4 h after irradiation, and it returned to the pre-irradiation level 24 to 48 h after the irradiation. The enzymatic activity of APN/CD13 was temporarily enhanced after irradiation injury, which might be one of the compensatory mechanisms that promote the hematopoietic recovery after irradiation.

摘要

本研究旨在探讨胚胎内主动脉-性腺-中肾（AGM）区基质细胞上氨肽酶N（APN）/CD13的表达情况，以及辐射损伤后其酶活性的变化。采用逆转录-聚合酶链反应（RT-PCR）和免疫组织化学方法检测AGM区基质细胞上APN/CD13的表达。用8.0 Gy的钴-60γ射线照射AGM区基质细胞后，在不同时间点用分光光度计测量APN/CD13的酶活性。结果显示，AGM区基质细胞强烈表达APN/CD13。辐射损伤后，APN/CD13的酶活性暂时降低，照射后4小时升高至较高水平，照射后24至48小时恢复到照射前水平。辐射损伤后APN/CD13的酶活性暂时增强，这可能是促进辐射后造血恢复的代偿机制之一。