HBx基因对肝细胞凋亡的影响。
The effect of HBx gene on the apoptosis of hepatic cells.
作者信息
Ye Lu, Qi Junying, Li Gaopeng, Tao Deding, Song Shihui
机构信息
Department of Infection, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology, Wuhan, China.
出版信息
J Huazhong Univ Sci Technolog Med Sci. 2007 Apr;27(2):167-9. doi: 10.1007/s11596-007-0215-x.
To study the effect of HBx gene on the apoptosis of the cell lines (L02, HepG2) and the interaction between HBx and X-linked inhibitor of apoptosis protein (XIAP), the apoptosis of pcDNA3.1-HBx transiently transfected cell lines (L02, HepG2) was detected by flow cytometry and the mRNA expression of XIAP was assayed by real-time RT-PCR. Our study showed (1) the morphology of L02/pcDNA3.1-HBx was changed and the appearance of the cells mimicked that of HepG2 cells; (2) HBx gene could be detected in L02/pcDNA3.1-HBx and HepG2/ pcDNA3.1-HBx; (3) the apoptosis rate of L02/pcDNA 3.1-HBx was higher than that of L02 cells (P<0.01) and the apoptosis rate of HepG2/pcDNA3.1-HBx was lower than that of HepG2 cells (P<0.05); (4) the XIAP expression in L02 was about 3 times that in L02/pcDNA3.1-HBx cells (P<0.01), and the expression of XIAP in HepG2/pcDNA3.1-HBx was about 4 times that in HepG2 (P<0.01). It is concluded that HBx gene may promote the apoptosis of normal hepatocytes and inhibit the apoptosis of cells of hepatic carcinoma by regulating the expression of XIAP.
为研究HBx基因对细胞系（L02、HepG2）凋亡的影响以及HBx与凋亡抑制蛋白X连锁抑制因子（XIAP）之间的相互作用，采用流式细胞术检测pcDNA3.1-HBx瞬时转染细胞系（L02、HepG2）的凋亡情况，并通过实时逆转录聚合酶链反应（RT-PCR）检测XIAP的mRNA表达。我们的研究表明：（1）L02/pcDNA3.1-HBx的形态发生改变，细胞外观类似HepG2细胞；（2）在L02/pcDNA3.1-HBx和HepG2/pcDNA3.1-HBx中可检测到HBx基因；（3）L02/pcDNA 3.1-HBx的凋亡率高于L02细胞（P<0.01），HepG2/pcDNA3.1-HBx的凋亡率低于HepG2细胞（P<0.05）；（4）L02中XIAP的表达约为L02/pcDNA3.1-HBx细胞中的3倍（P<0.01），HepG2/pcDNA3.1-HBx中XIAP的表达约为HepG2中的4倍（P<0.01）。结论是，HBx基因可能通过调节XIAP的表达促进正常肝细胞凋亡并抑制肝癌细胞凋亡。
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