Souguir Z, Roudesli S, About-Jaudet E, Le Cerf D, Picton L
UMR 6522 CNRS, Université de Rouen, Mont Saint Aignan 76821 Cedex, France.
J Colloid Interface Sci. 2007 Sep 1;313(1):108-16. doi: 10.1016/j.jcis.2007.03.012. Epub 2007 Mar 15.
Pullulan derivative was synthesized by coupling carboxymethylpullulan (degree of substitution DS(0)(in)=0.7) and dimethylaminopropylamine (DMAPA), activated by a hydrosoluble carbodiimide N(')-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC). FTIR and (13)C and (1)H NMR spectroscopic analyses have evidenced that the polysaccharide has been successfully modified. (1)H NMR, nitrogen analysis, and conductimetric measurements allow determination of the degree of substitution in both carboxylic acid and amine functions. We have found that both functions present a similar DS of 0.35, which is characteristic of an ampholytic polymer with possible zwitterionic-type properties. Solution properties have been studied by flow field flow fractionation (F4) coupled on-line with multiangle laser light scattering (MALLS) and quasi elastic light scattering (QELS), surface tension, and viscosity measurements. The behavior has been found largely pH dependent and an amphiphilic behavior has been evidenced in alkaline media.
通过将羧甲基普鲁兰多糖（取代度DS(0)(in)=0.7）与二甲基氨基丙胺（DMAPA）偶联，并使用水溶性碳二亚胺N'-(3-二甲氨基丙基)-N-乙基碳二亚胺盐酸盐（EDAC）进行活化，合成了普鲁兰多糖衍生物。傅里叶变换红外光谱（FTIR）、碳-13（(13)C）和氢-1（(1)H）核磁共振光谱分析证明该多糖已成功改性。氢-1核磁共振光谱、氮分析和电导率测量可确定羧酸和胺官能团的取代度。我们发现这两种官能团的取代度均为0.35左右，这是具有可能的两性离子型性质的两性聚合物的特征。通过与多角度激光光散射（MALLS）和准弹性光散射（QELS）在线联用的流场流分级（F4）、表面张力和粘度测量，研究了溶液性质。发现该行为在很大程度上依赖于pH值，并且在碱性介质中表现出两亲性行为。
