用于测定人血浆中喹硫平的经过验证的高效液相色谱-串联质谱法。

Validated HPLC-MS/MS method for determination of quetiapine in human plasma.

作者信息

Barrett B, Holcapek M, Huclová J, Borek-Dohalský V, Fejt P, Nemec B, Jelínek I

机构信息

Department of Bioanalytical Laboratory, CEPHA s.r.o. Plzen, Komenskeho 19, 32300 Plzen, Czech Republic.

出版信息

J Pharm Biomed Anal. 2007 Jun 28;44(2):498-505. doi: 10.1016/j.jpba.2007.03.034. Epub 2007 Apr 4.

DOI:10.1016/j.jpba.2007.03.034

PMID:17499470

Abstract

A validated, highly sensitive and selective high-pressure liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantitative determination of quetiapine (QUE) in human Na2EDTA plasma with mass spectrometry (MS) detection. Clozapine (CLO) was employed as an internal standard. Samples were extracted using solid phase extraction (SPE). Oasis HLB cartridges and the concentration of quetiapine was determined by isocratic HPLC-MS/MS. The SRM mode was used for MS/MS detection. The method was validated over a concentration range of 1.0-382.2 ng/mL. Inter- and intra-day precision and accuracy of the proposed method were characterized by relative standard deviation (R.S.D.) and the percentage of deviation, respectively; both were lower than 8%. The developed method was employed in the pharmacokinetic study of quetiapine.

摘要

建立了一种经过验证的、高灵敏度和高选择性的高效液相色谱-串联质谱（LC-MS/MS）方法，用于通过质谱（MS）检测定量测定人Na2EDTA血浆中的喹硫平（QUE）。使用氯氮平（CLO）作为内标。样品采用固相萃取（SPE）进行提取。使用Oasis HLB柱，并通过等度HPLC-MS/MS测定喹硫平的浓度。MS/MS检测采用SRM模式。该方法在1.0-382.2 ng/mL的浓度范围内进行了验证。所提出方法的日间和日内精密度及准确度分别通过相对标准偏差（R.S.D.）和偏差百分比来表征；两者均低于8%。所建立的方法用于喹硫平的药代动力学研究。