脂肪酶在无溶剂体系和乙腈中催化共轭亚油酸与左旋肉碱的酯化反应。

Lipase-catalyzed esterification of conjugated linoleic acid with L-carnitine in solvent-free system and acetonitrile.

作者信息

Li Zhen, Yang Depo, Jiang Lin, Ji Junfeng, Ji Hongbin, Zeng Xiaohui

机构信息

Lab of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, 510080, People's Republic of China.

出版信息

Bioprocess Biosyst Eng. 2007 Sep;30(5):331-6. doi: 10.1007/s00449-007-0129-9. Epub 2007 May 15.

DOI:10.1007/s00449-007-0129-9

PMID:17503088

Abstract

Lipase-catalyzed esterification of conjugated linoleic acid (CLA) with L-carnitine in solvent-free system and acetonitrile was studied. Three lipases (Novzym 435, Amamo AY30 and Amano AYS) have been assayed as suitable biocatalysts in the reaction. It was found that Amano AY30 was the most effective biocatalyst in both solvent-free system and acetonitrile. The conversion rate varied from 8.05 to 60.9% in terms of reaction conditions such as the amount of lipase, the presence of water, the amount of molecular sieves and reaction time. The conversions of substrate in solvent-free system were higher than that in acetonitrile. When the substrates were 1 mmol CLA and 1 mmol L-carnitine, the maximum conversion (60.9%) was obtained in solvent-free system with 150 mg lipase AY30, 50% water content and 150 mg molecular sieves at the reaction time of 24 h. A novel CLA ester product was successfully isolated and characterized by ESI-MS and (1)H NMR.

摘要

研究了在无溶剂体系和乙腈中脂肪酶催化共轭亚油酸（CLA）与L-肉碱的酯化反应。已测定三种脂肪酶（诺维信435、天野AY30和天野AYS）作为该反应合适的生物催化剂。结果发现，天野AY30在无溶剂体系和乙腈中都是最有效的生物催化剂。根据脂肪酶用量、水的存在、分子筛用量和反应时间等反应条件，转化率在8.05%至60.9%之间变化。无溶剂体系中底物的转化率高于乙腈中的转化率。当底物为1 mmol CLA和1 mmol L-肉碱时，在无溶剂体系中，加入150 mg脂肪酶AY30、含水量50%和150 mg分子筛，反应24 h时可获得最大转化率（60.9%）。成功分离出一种新型CLA酯产物，并通过电喷雾电离质谱（ESI-MS）和核磁共振氢谱（¹H NMR）对其进行了表征。