Rodrigues Thaisângela L S, Marchesan Julie T, Coletta Ricardo D, Novaes Arthur B, Grisi Márcio F de M, Souza Sérgio L S, Taba Mário, Palioto Daniela B
Department of Bucco-Maxillo-Facial Surgery and Traumatology and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
J Clin Periodontol. 2007 Jun;34(6):514-22. doi: 10.1111/j.1600-051X.2007.01090.x.
The objective of this study was to evaluate the effects of enamel matrix derivative (EMD), transforming growth factor-beta1 (TGF-beta1), and a combination of both factors (EMD+TGF-beta1) on periodontal ligament (PDL) fibroblasts.
Human PDL fibroblasts were obtained from three adult patients with a clinically healthy periodontium, using the explant technique. The effects of EMD, TGF-beta1, or a combination of both were analysed on PDL cell proliferation, adhesion, wound healing, and total protein synthesis, and on alkaline phosphatase (ALP) activity and bone-like nodule formation.
Treatment with EMD for 4, 7, and 10 days increased cell proliferation significantly compared with the negative control (p<0.05). At day 10, EMD and EMD+TGF-beta1 showed a higher cell proliferation compared with TGF-beta1 (p<0.01). Cell adhesion was significantly up-regulated by TGF-beta1 compared with EMD and EMD+TGF-beta1 (p<0.01). EMD enhanced in vitro wound healing of PDL cells compared with the other treatments. Total protein synthesis was significantly increased in PDL cells cultured with EMD compared with PDL cells treated with TGF-beta1 or EMD+TGF-beta1 (p<0.05). EMD induced ALP activity in PDL fibroblasts, which was associated with an increase of bone-like nodules.
These findings support the hypothesis that EMD and TGF-beta1 may play an important role in periodontal regeneration. EMD induced PDL fibroblast proliferation and migration, total protein synthesis, ALP activity, and mineralization, while TGF-beta1 increased cellular adhesion. However, the combination of both factors did not positively alter PDL fibroblast behaviour.
本研究的目的是评估釉基质衍生物（EMD）、转化生长因子-β1（TGF-β1）以及两者联合（EMD+TGF-β1）对牙周膜（PDL）成纤维细胞的影响。
采用组织块培养技术，从三名临床牙周健康的成年患者获取人PDL成纤维细胞。分析EMD、TGF-β1或两者联合对PDL细胞增殖、黏附、伤口愈合、总蛋白合成以及碱性磷酸酶（ALP）活性和类骨结节形成的影响。
与阴性对照相比，用EMD处理4天、7天和10天可显著增加细胞增殖（p<0.05）。在第10天，与TGF-β1相比，EMD和EMD+TGF-β1显示出更高的细胞增殖（p<0.01）。与EMD和EMD+TGF-β1相比，TGF-β1显著上调细胞黏附（p<0.01）。与其他处理相比，EMD增强了PDL细胞的体外伤口愈合。与用TGF-β1或EMD+TGF-β1处理的PDL细胞相比，用EMD培养的PDL细胞总蛋白合成显著增加（p<0.05）。EMD诱导PDL成纤维细胞中的ALP活性，这与类骨结节的增加有关。
这些发现支持以下假设，即EMD和TGF-β1可能在牙周再生中起重要作用。EMD诱导PDL成纤维细胞增殖和迁移、总蛋白合成、ALP活性和矿化，而TGF-β1增加细胞黏附。然而，两种因子的联合并未正向改变PDL成纤维细胞的行为。
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