一种用于快速生物传感应用的集成介电泳石英晶体微天平（DEP-QCM）装置。
An integrated dielectrophoretic quartz crystal microbalance (DEP-QCM) device for rapid biosensing applications.
作者信息
Fatoyinbo Henry O, Hoettges Kai F, Reddy Subrayal M, Hughes Michael P
机构信息
Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, Surrey GU2 7XH, UK.
出版信息
Biosens Bioelectron. 2007 Sep 30;23(2):225-32. doi: 10.1016/j.bios.2007.04.002. Epub 2007 Apr 6.
A factor limiting the detection time of biological particles using a quartz crystal microbalance (QCM) system is the kinetics of the particles arriving within the sensing region of the crystal surface. A device has been developed which, for the first time, combines ac electro-kinetic particle manipulation with simultaneous acoustic sensing on an electrode surface. We have termed this device a dielectrophoretic quartz crystal microbalance (DEP-QCM). Particles within the system are rapidly driven by electro-hydrodynamic and dielectrophoretic forces on to the crystal surface. Frequency shift analysis of mass-loaded DEP-QCM, induced by fluid motion, has shown significant improvements in rates of detection based on particle concentration, with steady-state responses established by a factor of five times faster than other quartz crystal microbalance surface loading techniques described in the literature. Comparisons of the static fluid case for QCM devices revealed that particles with a concentration of less than 10(8) nano-spheres/ml could not be detected within a 1h time period when allowed to sediment.
使用石英晶体微天平（QCM）系统检测生物颗粒时，一个限制检测时间的因素是颗粒到达晶体表面传感区域的动力学过程。现已开发出一种设备，首次将交流电动粒子操控与电极表面的同步声学传感相结合。我们将此设备称为介电泳石英晶体微天平（DEP-QCM）。系统内的颗粒通过电流体动力学和介电泳力快速驱动到晶体表面。由流体运动引起的质量加载DEP-QCM的频移分析表明，基于颗粒浓度的检测速率有显著提高，其稳态响应的建立速度比文献中描述的其他石英晶体微天平表面加载技术快五倍。对QCM设备的静态流体情况进行比较发现，当允许浓度低于10⁸个纳米球/毫升的颗粒沉降时，在1小时内无法检测到它们。
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