以CD34作为肝祖细胞标志物对人胎儿肝脏中的肝祖细胞进行鉴定。
Characterization of hepatic progenitors from human fetal liver using CD34 as a hepatic progenitor marker.
作者信息
Nyamath Parveen, Alvi Ayesha, Habeeb Aejaz, Khosla Sanjeev, Khan Aleem A, Habibullah C M
机构信息
Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences and Allied Hospitals, Kanchanbagh 500058, Hyderabad-A.P, India.
出版信息
World J Gastroenterol. 2007 Apr 28;13(16):2319-23. doi: 10.3748/wjg.v13.i16.2319.
AIM
To enrich putative hepatic progenitors from the developing human fetal liver using CD34 as a marker.
METHODS
Aborted fetuses of 13-20 wk were used for the isolation of liver cells. The cells were labeled with anti CD34; a marker used for isolating progenitor population and the cells were sorted using magnetic cell sorting. The positive fractions of cells were assessed for specific hepatic markers. Further, these cells were cultured in vitro for long term investigation.
RESULTS
Flow cytometric and immunocytochemical analysis for alphafetoprotein (AFP) showed that the majority of the enriched CD34 positive cells were positive for AFP. Furthermore, these enriched cells proliferated in the long term and maintained hepatic characteristics in in vitro culture.
CONCLUSION
The study shows that aborted human fetal liver is a potential source for isolation of hepatic progenitors for clinical applications. The study also demonstrates that CD34 can be a good marker for the enrichment of progenitor populations.
目的
以CD34为标志物，从发育中的人类胎儿肝脏中富集假定的肝祖细胞。
方法
使用13 - 20周的流产胎儿分离肝细胞。细胞用抗CD34标记；CD34是一种用于分离祖细胞群体的标志物，然后使用磁性细胞分选法对细胞进行分选。对细胞的阳性部分进行特定肝标志物的评估。此外，将这些细胞进行体外长期培养研究。
结果
甲胎蛋白（AFP）的流式细胞术和免疫细胞化学分析表明，大多数富集的CD34阳性细胞AFP呈阳性。此外，这些富集的细胞长期增殖并在体外培养中保持肝脏特性。
结论
该研究表明，流产的人类胎儿肝脏是分离用于临床应用的肝祖细胞的潜在来源。该研究还证明，CD34可以作为富集祖细胞群体的良好标志物。
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