Lotem J, Shabo Y, Sachs L
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
Cell Growth Differ. 1991 Sep;2(9):421-7.
There are clones of myeloid leukemic cells that can be induced to undergo terminal cell differentiation to macrophages by normal hemopoietic regulatory proteins. Induction of differentiation in two different clones of myeloid leukemic cells with interleukin 6 (IL-6) or granulocyte-macrophage colony-stimulating factor (GM-CSF) resulted in induction of mRNA for the hemopoietic regulatory proteins IL-6, GM-CSF, interleukin 1 alpha and interleukin 1 beta, tumor necrosis factor, and transforming growth factor beta 1. In one of these clones, induction of differentiation with GM-CSF was also associated with induction of mRNA for macrophage colony-stimulating factor (M-CSF) but not for the receptor for M-CSF (c-fms), whereas in the other clone, induction of differentiation with IL-6 was associated with induction of mRNA for both c-fms and M-CSF. The clones also differed in their responsiveness to these regulators. There was no induction of mRNA for granulocyte colony-stimulating factor or interleukin 3 during differentiation of either clone. The results indicate that the genes for a nearly normal network of positive and negative hemopoietic regulatory proteins are induced during differentiation of these myeloid leukemic cells and that there are leukemic clones with specific defects in this network.
存在一些髓系白血病细胞克隆，它们可被正常造血调节蛋白诱导发生终末细胞分化成为巨噬细胞。用白细胞介素6（IL-6）或粒细胞-巨噬细胞集落刺激因子（GM-CSF）诱导两种不同的髓系白血病细胞克隆分化，会导致造血调节蛋白IL-6、GM-CSF、白细胞介素1α、白细胞介素1β、肿瘤坏死因子和转化生长因子β1的mRNA表达。在其中一个克隆中，用GM-CSF诱导分化还与巨噬细胞集落刺激因子（M-CSF）的mRNA表达诱导相关，但与M-CSF受体（c-fms）的mRNA表达无关，而在另一个克隆中，用IL-6诱导分化与c-fms和M-CSF的mRNA表达诱导均相关。这些克隆对这些调节因子的反应性也有所不同。在任何一个克隆分化过程中，均未诱导粒细胞集落刺激因子或白细胞介素3的mRNA表达。结果表明，在这些髓系白血病细胞分化过程中，诱导了一个近乎正常的正负造血调节蛋白网络的基因，并且在这个网络中存在具有特定缺陷的白血病克隆。
