Güven Mehmet, Unal Mustafa, Sarici Ahmet, Ozaydin Ahmet, Batar Bahadir, Devranoğlu Kazim
Department of Medical Biology, Cerrahpasa Faculty of Medicine, University of Istanbul, Istanbul, Turkey.
Curr Eye Res. 2007 May;32(5):447-54. doi: 10.1080/02713680701338108.
In this study, we aimed to determine the effects of genetic polymorphisms of glutathione-S-transferase M1 (GSTM1) and glutathione-S-transferase T1 (GSTT1) on risk of developing different subtypes of age-related cataract in the Turkish population. Using a multiplex polymerase chain reaction (PCR), GSTM1 and GSTT1 gene polymorphisms were analyzed in 195 patients with age-related cataract (75 patients with cortical, 53 with nuclear, 37 with posterior subcapsular, and 30 with mixed type) and in 136 patients of an otherwise healthy control group of similar age. GSTM1 null genotype had a significant association with the development of cataract in female subjects (p < 0.0029; OR, 2.98; 95% CI, 1.41-6.34). This relationship in female subjects was only in nuclear and mixed types cataract cases (p < 0.002; OR, 4.58; 95% CI, 1.67-12.78 and p < 0.03, respectively). There was also a statistically significant association between the combination of GSTM1-null and GSTT1-positive genotypes and the risk of cataract development in female subjects (p = 0.01; OR = 2.87; 95% CI = 1.25-6.69). Stratification by the subtypes revealed that this association was only in nuclear type cataract (p = 0.001; OR, 3.92; 95% CI, 1.34-11.71). GSTM1-null genotype or combination of the GSTM1-null and GSTT1-positive genotypes in females may be associated with increased risk of cataract development in the Turkish population.
在本研究中，我们旨在确定谷胱甘肽 - S - 转移酶M1（GSTM1）和谷胱甘肽 - S - 转移酶T1（GSTT1）的基因多态性对土耳其人群中不同亚型年龄相关性白内障发病风险的影响。使用多重聚合酶链反应（PCR），对195例年龄相关性白内障患者（75例皮质性白内障、53例核性白内障、37例后囊下白内障和30例混合型白内障）以及136例年龄相仿的健康对照组患者进行了GSTM1和GSTT1基因多态性分析。GSTM1无效基因型与女性受试者白内障的发生显著相关（p < 0.0029；OR，2.98；95% CI，1.41 - 6.34）。女性受试者中的这种关系仅存在于核性和混合型白内障病例中（分别为p < 0.002；OR，4.58；95% CI，1.67 - 12.78和p < 0.03）。GSTM1无效基因型与GSTT1阳性基因型的组合与女性受试者白内障发生风险之间也存在统计学显著关联（p = 0.01；OR = 2.87；95% CI = 1.25 - 6.69）。按亚型分层显示，这种关联仅存在于核性白内障中（p = 0.001；OR，3.92；95% CI，1.34 - 11.71）。女性中的GSTM1无效基因型或GSTM1无效与GSTT1阳性基因型的组合可能与土耳其人群中白内障发生风险增加有关。
