[诺帝对人恶性胶质瘤细胞系U87趋化因子受体功能的影响]

[Effect of nordy on FPR function of malignant human glioma cell line U87].

作者信息

Chen Jian-Hong, Bian Xiu-Wu, Yao Xiao-Hong, Yang Shi-Xin, Xu Chang-Rong, Zhou Xiang-Dong, Ping Yi-Fang

机构信息

Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.

出版信息

Yao Xue Xue Bao. 2007 Mar;42(3):257-62.

PMID:17520823

Abstract

Nordy is a synthesized chrial compound. To investigate the effects of nordy (25 - 100 micromol x L(-1)) on the function of formylpeptide receptor (FPR) of malignant human glioma cells, human glioblastoma cell line U87 was used to detect its proliferation, migration, calcium mobilization, vascular endothelial growth factor (VEGF) mRNA and protein levels after activation of FPR by its agonist N-formyl-methionyl-leucyl-phenylalanine (fMLF). Cell proliferation, migration ability, VEGF mRNA, VEGF protein and calcium mobilization were evaluated by cell counting, chemotaxis assay, RT-PCR, ELISA and spectrometry. Nordy (50 - 100 micromol x L(-1)) potently inhibited the proliferation, migration and calcium mobilization of U87 cells induced by fMLF (P < 0.05). Moreover, 100 micromol x L(-1) nordy showed a significantly impaired VEGF mRNA expression and protein secretion induced by fMLF (P < 0.05). Nordy could inhibit FPR functioning in glioma cell proliferation, migration and angiogenesis, which might be a possible mechanism of its anti-cancer effects.

摘要

诺帝是一种合成的手性化合物。为研究诺帝（25 - 100微摩尔×升⁻¹）对人恶性胶质瘤细胞甲酰肽受体（FPR）功能的影响，采用人胶质母细胞瘤细胞系U87，检测其在激动剂N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLF）激活FPR后的增殖、迁移、钙动员、血管内皮生长因子（VEGF）mRNA和蛋白水平。通过细胞计数、趋化性分析、逆转录-聚合酶链反应（RT-PCR）、酶联免疫吸附测定（ELISA）和光谱法评估细胞增殖、迁移能力、VEGF mRNA、VEGF蛋白和钙动员情况。诺帝（50 - 100微摩尔×升⁻¹）有效抑制了fMLF诱导的U87细胞增殖、迁移和钙动员（P < 0.05）。此外，100微摩尔×升⁻¹诺帝显著削弱了fMLF诱导的VEGF mRNA表达和蛋白分泌（P < 0.05）。诺帝可抑制胶质瘤细胞增殖、迁移和血管生成过程中FPR的功能，这可能是其抗癌作用的一种潜在机制。