使用二维液相色谱/串联质谱法鉴定人子宫内膜癌细胞和宫颈癌细胞释放的候选生物标志物蛋白。
Identification of candidate biomarker proteins released by human endometrial and cervical cancer cells using two-dimensional liquid chromatography/tandem mass spectrometry.
作者信息
Li Hongyan, DeSouza Leroi V, Ghanny Shaun, Li Wei, Romaschin Alexander D, Colgan Terence J, Siu K W Michael
机构信息
Department of Biology, Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, Ontario, Canada.
出版信息
J Proteome Res. 2007 Jul;6(7):2615-22. doi: 10.1021/pr0700798. Epub 2007 May 25.
Candidate biomarker proteins, including chaperonin 10 and pyruvate kinase, previously discovered and identified using mass-tagging reagents with multidimensional liquid chromatography and tandem mass spectrometry (DeSouza, L.; et al. J. Proteome Res. 2005, 4, 377-386) have been identified in serum-free media of cultured endometrial cancer (KLE and HEC-1-A) and cervical cancer (HeLa) cells. These and other cancer-associated proteins were released by the cultured cells within 24 h of growth. A total of 203 proteins from the KLE cells, 86 from HEC-1-A, and 161 from HeLa are reported.
先前使用带有多维液相色谱和串联质谱的质量标记试剂发现并鉴定出的候选生物标志物蛋白，包括伴侣蛋白10和丙酮酸激酶（德索萨，L.等人，《蛋白质组研究杂志》，2005年，第4卷，第377 - 386页），已在培养的子宫内膜癌（KLE和HEC - 1 - A）和宫颈癌（HeLa）细胞的无血清培养基中被鉴定出来。这些以及其他与癌症相关的蛋白质在细胞生长24小时内由培养细胞释放出来。报告了来自KLE细胞的总共203种蛋白质、来自HEC - 1 - A的86种蛋白质以及来自HeLa的161种蛋白质。
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