铜（Ⅱ）-β-环糊精复合物与小牛胸腺DNA的结合

Binding of Cu(2+)-beta-cyclodextrin complex to calf thymus DNA.

作者信息

Divakar S, Ramasarma P R, Maheswaran M M

机构信息

Organic Chemistry Section, Central Food Technological Research Institute, Mysore, India.

出版信息

Indian J Biochem Biophys. 1991 Aug;28(4):247-51.

PMID:1752626

Abstract

Cu(2+)-beta-cyclodextrin (1:1) complex has been found by UV, fluorescence and CD spectroscopy, polarimetry and gel electrophoresis to bind reversibly to calf thymus DNA. Using UV the binding constant was found to be 45280 +/- 7100 M-1. The binding of the complex Cu(2+)-BCD with DNA was stronger than that of free Cu2+. However the ternary complex formed thus was destroyed by EDTA.

摘要

通过紫外光谱、荧光光谱、圆二色光谱、旋光测定法和凝胶电泳发现，铜（Ⅱ）-β-环糊精（1:1）络合物能与小牛胸腺DNA可逆结合。利用紫外光谱测得结合常数为45280±7100 M⁻¹。铜（Ⅱ）-β-环糊精络合物与DNA的结合比游离铜离子更强。然而，由此形成的三元络合物会被乙二胺四乙酸破坏。