Roszczenko Paula, Jagusztyn-Krynicka Elzbieta Katarzyna
Zakład Genetyki Bakterii, Instytut biologii, Wydział Biologii, Uniwersytet Warszawski, Warszawa.
Postepy Biochem. 2006;52(4):424-34.
Helicobacter pylori, Gram-negative spiral-shaped bacteria, member of epsilon-Proteobacteria, colonizes the gastric mucosa of humans. H. pylori has been identified as the causative agent of chronic inflammation, chronic gastritis and peptic ulceration and is considered a risk factor for the development of mucosa-associated lymphoid tissue lymphoma and adenocarcinoma of the stomach. Although more than 50% of human population is infected with H. pylori only a subset develops disease. The completion of two H. pylori genome sequences revealed the enormous strain heterogeneity and permitted comparative proteome analysis. Immunoproteomics, a novel strategy combining standard proteomics with immunological screening, is currently method of choice for identification of new antigens of diagnostic and protective values. Highly specific antigens will be used as biomarkers of different pathology induced by H. pylori infection whereas novel highly immunogenic, conserved, abundant and surface-located proteins will facilitate efficient anti-Helicobacter vaccine construction.
幽门螺杆菌是革兰氏阴性螺旋形细菌，属于ε-变形菌纲，定植于人类胃黏膜。幽门螺杆菌已被确认为慢性炎症、慢性胃炎和消化性溃疡的病原体，并被认为是黏膜相关淋巴组织淋巴瘤和胃癌发生的危险因素。尽管超过50%的人群感染了幽门螺杆菌，但只有一部分人会发病。两个幽门螺杆菌基因组序列的完成揭示了巨大的菌株异质性，并允许进行比较蛋白质组分析。免疫蛋白质组学是一种将标准蛋白质组学与免疫筛选相结合的新策略，目前是鉴定具有诊断和保护价值的新抗原的首选方法。高度特异性抗原将用作幽门螺杆菌感染诱导的不同病理的生物标志物，而新型的高免疫原性、保守、丰富且位于表面的蛋白质将有助于高效构建抗幽门螺杆菌疫苗。
