Sabu Sahadevan, Yang Fu-Chia, Wang Yi-Sheng, Chen Wei-Hao, Chou Meng-Ing, Chang Huan-Cheng, Han Chau-Chung
Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
Anal Biochem. 2007 Aug 15;367(2):190-200. doi: 10.1016/j.ab.2007.04.033. Epub 2007 Apr 27.
Electrospray ionization (ESI) has been an indispensable ion generation technique for mass spectrometric analysis of biopolymers such as intact proteins and protein digests operated at atmospheric pressure. Since its advent in 1998, atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) quickly became a popular alternative for the analysis of peptides. Although AP-MALDI sources typically share the same vacuum interface and ion transmission hardware with ESI, it is generally found that ESI is superior in detection sensitivity. Here we present a method based on solid phase extraction and elution with surface-functionalized diamond nanocrystals (which we previously referred to as "SPEED") that not only streamlines AP-MALDI mass spectrometric analyses of peptides and other small biomolecules under typical operational conditions but also outruns ESI in ultimate detectable concentration by at least one order of magnitude.
电喷雾电离（ESI）一直是用于在大气压下对生物聚合物（如完整蛋白质和蛋白质消化产物）进行质谱分析的不可或缺的离子产生技术。自1998年问世以来，大气压基质辅助激光解吸/电离（AP-MALDI）迅速成为分析肽的一种流行替代方法。尽管AP-MALDI源通常与ESI共享相同的真空接口和离子传输硬件，但一般发现ESI在检测灵敏度方面更具优势。在此，我们提出一种基于用表面功能化金刚石纳米晶体进行固相萃取和洗脱的方法（我们之前称之为“SPEED”），该方法不仅简化了在典型操作条件下对肽和其他小生物分子的AP-MALDI质谱分析，而且在最终可检测浓度方面比ESI至少高出一个数量级。
