Stabellini G, Calvitti M, Becchetti E, Carinci P, Calastrini C, Lilli C, Solmi R, Vizzotto L, Baroni T
Human Morphology Department, University of Milano, Milan, Italy.
Eur J Histochem. 2007 Jan-Mar;51(1):33-41.
Normal branching development is dependent on the correlation between cells and extracellular matrix. In this interaction glycosaminoglycans, cytokines and growth factors play a fundamental role. In order to verify the distribution and influence of extracellular matrix and related enzymes on chick embryo lung development, 6 day-old whole lungs were maintained in vitro with testicular hyaluronidase, beta-N-acetyl-D-glucosaminidase and chondrotinase ABC or in linkage with apical, medial and caudal lung regions of 6-day development before and after enzyme treatment. In a separate lung region beta-N-acetyl-D-glucosaminidase and hyaluronidase were determined. Our data show that the whole lung cultures increase bronchial branching development when the medial region is admixed separately, while the separate apical or caudal regions or apical combined with caudal region do not affect bronchial branching development. The enzyme treatment of medial region prevents the branching development in associated whole lung. The bronchial branching development of whole lung cultured in medium containing the enzymes related to glycosaminoglycans turnover is significantly altered. In conclusion, these data show that the different influence of separate apical, medial, caudal lung regions on bronchial branching development is related to the extracellular matrix composition.
正常的分支发育依赖于细胞与细胞外基质之间的相互关系。在这种相互作用中，糖胺聚糖、细胞因子和生长因子发挥着重要作用。为了验证细胞外基质及相关酶对鸡胚肺发育的分布和影响，将6日龄的全肺在体外与睾丸透明质酸酶、β-N-乙酰-D-氨基葡萄糖苷酶和软骨素酶ABC一起培养，或在酶处理前后与6日龄发育的肺尖、肺中和肺尾区域相连培养。在一个单独的肺区域测定β-N-乙酰-D-氨基葡萄糖苷酶和透明质酸酶。我们的数据表明，当肺中区域单独混合时，全肺培养物可增加支气管分支发育，而单独的肺尖或肺尾区域或肺尖与肺尾区域组合则不影响支气管分支发育。对肺中区域进行酶处理会阻止相关全肺的分支发育。在含有与糖胺聚糖代谢相关酶的培养基中培养的全肺的支气管分支发育会发生显著改变。总之，这些数据表明，肺尖、肺中、肺尾区域对支气管分支发育的不同影响与细胞外基质组成有关。
