Chen Wei-Zhi, Liu Chuan-Fang, Li Li-Zhen, Yin Xiao-Xiao
Department of Hematology, Qilu Hospital of Shandong University, Jinan 250012, China.
Zhonghua Yi Xue Za Zhi. 2007 Mar 13;87(10):714-6.
To investigate the highly specific proteasomal inhibitor MG132-induced apoptosis and its effect on nuclear factor (NF)-kappaB activation and survivin expression in leukemic K562 cell line.
leukemic cells of the line K562 were cultured and divided into 2 groups: treatment group, undergoing co-incubation with MG132 of the concentrations of 2, 4, 6, and 8 micromol/L respectively for 24 hours, and control group without treatment of MG132. Apoptosis was detected by examination of cell morphology and flow cytometry. Survivin expression and NF-kappaB activation were analyzed by immunocytochemistry and Western blotting.
MG132 induced apoptosis of the K562 cells dose-dependently. Both survivin and NF-kappaB were highly expressed in the K562 cells. Compared with the control group, K562 cell treated with MG132 at the concentrations of 2, 4, 6, and 8 micromol/L for 24 hours showed the decrease of NF-kappaB activation to 75.0% +/- 3.7%, 59.9% +/- 5.3%, 45.4% +/- 5.7%, and 25.0% +/- 4.2% respectively, and decrease of survivin expression to 90.9% +/- 10.1%, 66.7% +/- 5.2%, 45.4% +/- 5.7%, and 30.3% +/- 6.6% respectively. Downregulation of survivin expression was closely correlated with the inhibition of NF-kappaB activation (Pearson correlation coefficient = 0.989, P < 0.01).
MG132 induces apoptosis of leukemic cells, and effectively inhibits the NF-kappaB activation accompanied by the downregulation of survivin expression.
研究高特异性蛋白酶体抑制剂MG132诱导白血病K562细胞系凋亡及其对核因子（NF）-κB激活和生存素表达的影响。
培养白血病K562细胞系，分为2组：处理组分别用浓度为2、4、6和8 μmol/L的MG132共孵育24小时，对照组不进行MG132处理。通过细胞形态学检查和流式细胞术检测凋亡情况。通过免疫细胞化学和蛋白质印迹分析生存素表达和NF-κB激活情况。
MG132剂量依赖性地诱导K562细胞凋亡。生存素和NF-κB在K562细胞中均高表达。与对照组相比，用浓度为2、4、6和8 μmol/L的MG132处理24小时的K562细胞，NF-κB激活分别降至75.0%±3.7%、59.9%±5.3%、45.4%±5.7%和
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