Santos André L S, d'Avila-Levy Claudia M, Elias Camila G R, Vermelho Alane B, Branquinha Marta H
Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes (IMPPG), Centro de Ciências da Saúde (CCS), Bloco I, Universidade Federal do Rio de Janeiro (UFRJ), RJ 21941-590, Brazil.
Microbes Infect. 2007 Jul;9(8):915-21. doi: 10.1016/j.micinf.2007.03.018. Epub 2007 Apr 12.
The present review provides an overview of recent discoveries concerning the immunological similarities between Phytomonas serpens, a tomato parasite, and human trypanosomatid pathogens, with special emphasis on peptidases. Leishmania spp. and Trypanosoma cruzi express peptidases that are well-known virulence factors, named leishmanolysin and cruzipain. P. serpens synthesizes two distinct classes of proteolytic enzymes, metallo- and cysteine-type peptidases, that share common epitopes with leishmanolysin and cruzipain, respectively. The leishmanolysin-like and cruzipain-like molecules from P. serpens participate in several biological processes including cellular growth and adhesion to the salivary glands of Oncopeltus fasciatus, a phytophagous insect experimental model. Since previous reports demonstrated that immunization of mice with P. serpens induced a partial protective immune response against T. cruzi, this plant trypanosomatid may be a suitable candidate for vaccine studies. Moreover, comparative approaches in the Trypanosomatidae family may be useful to understand kinetoplastid biology, biochemistry and evolution.
本综述概述了有关番茄寄生虫丝虫（Phytomonas serpens）与人类锥虫病原体之间免疫相似性的最新发现，特别强调了肽酶。利什曼原虫属（Leishmania spp.）和克氏锥虫（Trypanosoma cruzi）表达的肽酶是众所周知的毒力因子，分别称为利什曼溶素和克氏锥虫蛋白酶。丝虫合成了两类不同的蛋白水解酶，金属肽酶和半胱氨酸型肽酶，它们分别与利什曼溶素和克氏锥虫蛋白酶具有共同的表位。丝虫的利什曼溶素样和克氏锥虫蛋白酶样分子参与多种生物学过程，包括细胞生长以及与植食性昆虫实验模型豆长蝽（Oncopeltus fasciatus）唾液腺的黏附。由于先前的报告表明用丝虫免疫小鼠可诱导对克氏锥虫的部分保护性免疫反应，这种植物锥虫可能是疫苗研究的合适候选者。此外，锥虫科中的比较方法可能有助于理解动基体生物学、生物化学和进化。
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