Murakami Kazunori, Enkhbaatar Perenlei, Yu Yong-Ming, Traber Lillian D, Cox Robert A, Hawkins Hal K, Tompkins Ronald G, Herndon David, Traber Daniel L
Department of Anesthesiology, University of Texas Medical Branch, TX 77555-0833, USA.
Shock. 2007 Oct;28(4):477-83. doi: 10.1097/shk.0b013e31804a59bd.
Thermal injury results in reduced plasma levels of arginine (Arg). With reduced Arg availability, NOS produces superoxide instead of NO. We hypothesized that Arg supplementation after burn and smoke inhalation (B + S) injury would attenuate the acute insult to the lungs and, thus, protect pulmonary function. Seventeen Suffolk ewes (n = 17) were randomly divided into three groups: (1) sham injury group (n = 6), (2) B + S injury plus saline treatment (n = 6), and (3) B + S injury plus L-ARG infusion at 57 mg.kg(-1).h(-1) (n = 5). Burn and smoke inhalation injury was induced by standardized procedures, including a 40% area full thickness flame burn combined with 48 breaths of smoke from burning cottons. All animals were immediately resuscitated by Ringer solution and supported by mechanical ventilation for 48 h, during which various variables of pulmonary function were monitored. The results demonstrated that Arg treatment attenuated the decline of plasma Arg concentration after B + S injury. A higher plasma Arg concentration was associated with a less decline in Pao2/Fio2 ratio and a reduced extent of airway obstruction after B + S injury. Histopathological examinations also indicated a remarkably reduced histopathological scores associated with B + S injury. Nitrotyrosine stain in lung tissue was positive after B + S injury, but was significantly reduced in the group with Arg. Therefore, L-Arg supplementation improved gas exchange and pulmonary function in ovine after B + S injury via its, at least in part, effect on reduction of oxidative stress through the peroxynitrite pathway.
热损伤会导致血浆中精氨酸（Arg）水平降低。随着精氨酸可用性的降低，一氧化氮合酶（NOS）产生的是超氧化物而不是一氧化氮（NO）。我们推测，在烧伤和烟雾吸入（B + S）损伤后补充精氨酸会减轻对肺部的急性损伤，从而保护肺功能。17只萨福克母羊（n = 17）被随机分为三组：（1）假损伤组（n = 6），（2）B + S损伤加生理盐水治疗组（n = 6），以及（3）B + S损伤加57 mg·kg⁻¹·h⁻¹的L - 精氨酸输注组（n = 5）。通过标准化程序诱导烧伤和烟雾吸入损伤，包括40%体表面积的全层火焰烧伤并结合48次吸入燃烧棉花产生的烟雾。所有动物立即用林格氏液进行复苏，并通过机械通气支持48小时，在此期间监测各种肺功能变量。结果表明，精氨酸治疗减轻了B + S损伤后血浆精氨酸浓度的下降。较高的血浆精氨酸浓度与B + S损伤后Pao2/Fio2比值下降较少以及气道阻塞程度减轻相关。组织病理学检查还表明，与B + S损伤相关的组织病理学评分显著降低。B + S损伤后肺组织中的硝基酪氨酸染色呈阳性，但在精氨酸组中显著降低。因此，补充L - 精氨酸至少部分通过过氧亚硝酸盐途径减轻氧化应激，从而改善了B + S损伤后绵羊的气体交换和肺功能。
